ID   GLGB_SOLTU              Reviewed;         861 AA.
AC   P30924;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=1,4-alpha-glucan-branching enzyme;
DE            EC=2.4.1.18;
DE   AltName: Full=Q-enzyme;
DE   AltName: Full=Starch-branching enzyme;
GN   Name=SBE1; Synonyms=SBE;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Dianella;
RX   PubMed=8278528; DOI=10.1104/pp.102.3.1053;
RA   Poulsen P., Kreiberg J.D.;
RT   "Starch branching enzyme cDNA from Solanum tuberosum.";
RL   Plant Physiol. 102:1053-1054(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 279-527.
RC   STRAIN=cv. Desiree; TISSUE=Tuber;
RX   PubMed=1745241; DOI=10.1007/bf00290648;
RA   Kossmann J., Visser R.G.F., Mueller-Roeber B., Willmitzer L., Sonnewald U.;
RT   "Cloning and expression analysis of a potato cDNA that encodes branching
RT   enzyme: evidence for co-expression of starch biosynthetic genes.";
RL   Mol. Gen. Genet. 230:39-44(1991).
CC   -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC       in starch by scission of a 1,4-alpha-linked oligosaccharide from
CC       growing alpha-1,4-glucan chains and the subsequent attachment of the
CC       oligosaccharide to the alpha-1,6 position.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC         primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X69805; CAA49463.1; -; mRNA.
DR   PIR; S34730; S34730.
DR   RefSeq; NP_001275183.1; NM_001288254.1.
DR   AlphaFoldDB; P30924; -.
DR   SMR; P30924; -.
DR   STRING; 4113.PGSC0003DMT400025846; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   PRIDE; P30924; -.
DR   GeneID; 102596498; -.
DR   KEGG; sot:102596498; -.
DR   InParanoid; P30924; -.
DR   OrthoDB; 165238at2759; -.
DR   BioCyc; MetaCyc:MON-1885; -.
DR   UniPathway; UPA00152; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P30924; baseline and differential.
DR   GO; GO:0009501; C:amyloplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IBA:GO_Central.
DR   GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IEA:InterPro.
DR   GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   InterPro; IPR006048; A-amylase/branching_C.
DR   InterPro; IPR037439; Branching_enzy.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR43651; PTHR43651; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   PIRSF; PIRSF000463; GlgB; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
PE   2: Evidence at transcript level;
KW   Amyloplast; Chloroplast; Glycosyltransferase; Plastid; Reference proteome;
KW   Starch biosynthesis; Transferase.
FT   CHAIN           1..861
FT                   /note="1,4-alpha-glucan-branching enzyme"
FT                   /id="PRO_0000188787"
FT   ACT_SITE        429
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   861 AA;  99084 MW;  F3D519AC7CF1BEF2 CRC64;
     MEINFKVLSK PIRGSFPSFS PKVSSGASRN KICFPSQHST GLKFGSQERS WDISSTPKSR
     VRKDERMKHS SAISAVLTDD NSTMAPLEED VKTENIGLLN LDPTLEPYLD HFRHRMKRYV
     DQKMLIEKYE GPLEEFAQGY LKFGFNREDG CIVYREWAPA AQEDEVIGDF NGWNGSNHMM
     EKDQFGVWSI RIPDVDSKPV IPHNSRVKFR FKHGNGVWVD RIPAWIKYAT ADATKFAAPY
     DGVYWDPPPS ERYHFKYPRP PKPRAPRIYE AHVGMSSSEP RVNSYREFAD DVLPRIKANN
     YNTVQLMAIM EHSYYGSFGY HVTNFFAVSS RYGNPEDLKY LIDKAHSLGL QVLVDVVHSH
     ASNNVTDGLN GFDIGQGSQE SYFHAGERGY HKLWDSRLFN YANWEVLRFL LSNLRWWLEE
     YNFDGFRFDG ITSMLYVHHG INMGFTGNYN EYFSEATDVD AVVYLMLANN LIHKIFPDAT
     VIAEDVSGMP GLGRPVSEGG IGFDYRLAMA IPDKWIDYLK NKNDEDWSMK EVTSSLTNRR
     YTEKCIAYAE SHDQSIVGDK TIAFLLMDKE MYSGMSCLTD ASPVVDRGIA LHKMIHFFTM
     ALGGEGYLNF MGNEFGHPEW IDFPREGNNW SYDKCRRQWN LADSEHLRYK FMNAFDRAMN
     SLDEKFSFLA SGKQIVSSMD DDNKVVVFER GDLVFVFNFH PKNTYEGYKV GCDLPGKYRV
     ALDSDAWEFG GHGRTGHDVD HFTSPEGIPG VPETNFNGRQ IPSKCCLLRE HVWLITELMN
     ACQKLKITRQ TFVVSYYQQP ISRRVTRNLK IRYLQISVTL TNACQKLKFT RQTFLVSYYQ
     QPILRRVTRK LKDSLSTNIS T