ID   GLGB_SYNPW              Reviewed;         761 AA.
AC   A5GJR7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685};
DE            EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685};
DE   AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685};
DE   AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE   AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE            Short=BE {ECO:0000255|HAMAP-Rule:MF_00685};
GN   Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685};
GN   OrderedLocusNames=SynWH7803_0756;
OS   Synechococcus sp. (strain WH7803).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC   unclassified Synechococcus.
OX   NCBI_TaxID=32051;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WH7803;
RG   Genoscope;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC       in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC       growing alpha-1,4-glucan chains and the subsequent attachment of the
CC       oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-
CC       Rule:MF_00685}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC         primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
CC   -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00685}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}.
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DR   EMBL; CT971583; CAK23182.1; -; Genomic_DNA.
DR   RefSeq; WP_011932665.1; NC_009481.1.
DR   AlphaFoldDB; A5GJR7; -.
DR   SMR; A5GJR7; -.
DR   STRING; 32051.SynWH7803_0756; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   PRIDE; A5GJR7; -.
DR   EnsemblBacteria; CAK23182; CAK23182; SynWH7803_0756.
DR   KEGG; syx:SynWH7803_0756; -.
DR   eggNOG; COG0296; Bacteria.
DR   HOGENOM; CLU_004245_3_2_3; -.
DR   OMA; FGMKWMM; -.
DR   OrthoDB; 227746at2; -.
DR   UniPathway; UPA00164; -.
DR   Proteomes; UP000001566; Chromosome.
DR   GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd02855; E_set_GBE_prok_N; 1.
DR   Gene3D; 2.60.40.10; -; 2.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   HAMAP; MF_00685; GlgB; 1.
DR   InterPro; IPR006048; A-amylase/branching_C.
DR   InterPro; IPR037439; Branching_enzy.
DR   InterPro; IPR006407; GlgB.
DR   InterPro; IPR044143; GlgB_N_E_set_prok.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR43651; PTHR43651; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   Pfam; PF02922; CBM_48; 2.
DR   PIRSF; PIRSF000463; GlgB; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 2.
DR   TIGRFAMs; TIGR01515; branching_enzym; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..761
FT                   /note="1,4-alpha-glucan branching enzyme GlgB"
FT                   /id="PRO_1000045004"
FT   ACT_SITE        431
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
SQ   SEQUENCE   761 AA;  88001 MW;  BB022989B282244C CRC64;
     MTNTVLEWMV EDAQRLAECR HDHPFAVLGP QPQEDGTWVL RAWMPEAEGV TLLLGDQEMG
     MSTPHHPWIF EASVAHDPGC AYRLRVHRGG ITHEQHDPWA FRQDWMGEMD RHLFAEGNHH
     HIWRRMGAHH CEREGIPGVM FCLWAPHARS VSVIGDLNSW DGRHHPMQQR LGGIWELFVP
     GLAEGQLYKY EIRTQEGHCY QKADPYGFQH EVRPDTSSVV SHLDGFHWTD TDWIQKRDSS
     NPLDQPIAVY EMHLGSWIHA AADEPFIEAD GTPRAPVPAA DLKPGARLLT YPELADRLIP
     YVKERGFTHI ELMPITEHPF DGSWGYQVTG WYAPTSRYGT PDEFRAFVDR CHAEGIGVII
     DWVPGHFPRD SHGLAFFDGC HLYEHADPRI GEHKEWGTLI FNYSRNEVRN FLVANLVFWF
     DQFHIDGIRV DAVASMLYRD YLRPDGEWLP NEHGGRENTE AVQFLQQANH VLFQHFPGAL
     SIAEESTTWP MVTQPTDIGG LGFNLKWNMG WMHDMLDYFE LDPWFRQFHQ NNITFSIWYT
     YTENFMLALS HDEVVHGKSN LLHKMPGDDW QKYANTRALL AYMWTHPGKK TIFMGMEFGQ
     RAEWNVWGDL QWDLLNYEPH QGVQRMVDDL NGLYKSEPAL WRDDFDQYGF QWIDCNDNRH
     SVISFMRRES TSGTWLVVVA NFTPQSHSHY RVGVPLAGYY EEIFNTDASK YGGSNLGNMG
     GKPTEEWGIH GYENSLDLCL PPLSLMVFKH DPKKSLNPVQ E