GLGB_SYNY3
ID GLGB_SYNY3 Reviewed; 770 AA.
AC P52981;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB;
DE EC=2.4.1.18;
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase;
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme;
DE AltName: Full=Glycogen branching enzyme;
DE Short=BE;
GN Name=glgB; OrderedLocusNames=sll0158;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000022; BAA10073.1; -; Genomic_DNA.
DR PIR; S76095; S76095.
DR AlphaFoldDB; P52981; -.
DR SMR; P52981; -.
DR IntAct; P52981; 1.
DR STRING; 1148.1001448; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR PaxDb; P52981; -.
DR PRIDE; P52981; -.
DR EnsemblBacteria; BAA10073; BAA10073; BAA10073.
DR KEGG; syn:sll0158; -.
DR eggNOG; COG0296; Bacteria.
DR InParanoid; P52981; -.
DR OMA; FGMKWMM; -.
DR PhylomeDB; P52981; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IBA:GO_Central.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0005978; P:glycogen biosynthetic process; IBA:GO_Central.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 2.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..770
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000188758"
FT ACT_SITE 433
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 486
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 770 AA; 89528 MW; A435AFCA7703FA8A CRC64;
MTYTINADQV HQIVHNLHHD PFEVLGCHPL GDHGKVNQWV IRAYLPTAEA VTVLLPTDRR
EVIMTTVHHP NFFECVLELE EPKNYQLRIT ENGHERVIYD PYGFKTPKLT DFDLHVFGEG
NHHRIYEKLG AHLMTVDGVK GVYFAVWAPN ARNVSILGDF NNWDGRLHQM RKRNNMVWEL
FIPELGVGTS YKYEIKNWEG HIYEKTDPYG FYQEVRPKTA SIVADLDGYQ WHDEDWLEAR
RTSDPLSKPV SVYELHLGSW LHTAYDEPVK TLHGEGVPVE VSEWNTGARF LTYYELVDKL
IPYVKELGYT HIELLPIAEH PFDGSWGYQV TGYYAPTSRF GSPEDFMYFV DQCHLNGIGV
IIDWVPGHFP KDGHGLAFFD GTHLYEHGDP RKGEHKEWGT LIFNYGRNEV RNFLVANALF
WFDKYHIDGM RVDAVASMLY LDYCREEGEW VANEYGGREN LEAADFLRQV NSVVYSYFPG
ILSIAEESTS WPMVSWPTYV GGLGFNLKWN MGWMHDMLDY FSMDPWFRQF HQNSITFSMW
YNHSENYMLA LSHDEVVHGK SNMLGKMPGD EWQKYANVRA LFTYMFTHPG KKTMFMSMEF
GQWSEWNVWG DLEWHLLNFP PHQQLKQFFT ELNHLYKNEP ALYSNDFDES GFQWIDCSDN
RHSVVSFIRR AKNSAEFVVT ICNFTPQPHS HYRVGVPVPG FYTELFNSDA RQYGGSNMGN
LGGKWTEEWS FHEQPYSLDL CLPPLSVLVL KLSQNAEENT VPAEEASNIA
