3S11_MICLL
ID 3S11_MICLL Reviewed; 81 AA.
AC K9MCH1;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=Three-finger toxin A1 {ECO:0000305};
DE Short=MlatA1 {ECO:0000303|PubMed:23438486};
DE AltName: Full=Short chain alpha-neurotoxin A1 {ECO:0000303|PubMed:23438486};
DE Flags: Precursor;
OS Micrurus laticollaris (Balsas coral snake).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Micrurus.
OX NCBI_TaxID=1240351;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-51 AND 59-77,
RP SUBCELLULAR LOCATION, TOXIC DOSE, FUNCTION, AND MASS SPECTROMETRY.
RC TISSUE=Venom, and Venom gland;
RX PubMed=23438486; DOI=10.1016/j.toxicon.2013.02.006;
RA Carbajal-Saucedo A., Lopez-Vera E., Benard-Valle M., Smith E.N.,
RA Zamudio F., de Roodt A.R., Olvera-Rodriguez A.;
RT "Isolation, characterization, cloning and expression of an alpha-neurotoxin
RT from the venom of the Mexican coral snake Micrurus laticollaris (Squamata:
RT Elapidae).";
RL Toxicon 66:64-74(2013).
CC -!- FUNCTION: Binds and inhibits fetal (alpha-1-beta-1-gamma-delta/CHRNA1-
CC CHRNB1-CHRNG-CHRND, IC(50)=1.4 nM), adult (alpha-1-beta-1-delta-
CC epsilon/CHRNA1-CHRNB1-CHRND-CHRNE, IC(50)=12 nM) and neuronal alpha-
CC 7/CHRNA7 (IC(50)=400 nM) nicotinic acetylcholine receptors (nAChR)
CC thereby impairing neuromuscular and neuronal transmissions.
CC {ECO:0000269|PubMed:23438486}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23438486}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=6673.0; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:23438486};
CC -!- TOXIC DOSE: LD(50) is 63.5 ug/kg by intravenous injection into mice
CC (1.27 ug/mouse). {ECO:0000269|PubMed:23438486}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR EMBL; JQ247015; AFU76492.1; -; mRNA.
DR AlphaFoldDB; K9MCH1; -.
DR SMR; K9MCH1; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Neurotoxin; Postsynaptic neurotoxin; Secreted; Signal;
KW Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:23438486"
FT CHAIN 22..81
FT /note="Three-finger toxin A1"
FT /evidence="ECO:0000305|PubMed:23438486"
FT /id="PRO_0000430894"
FT DISULFID 24..43
FT /evidence="ECO:0000250|UniProtKB:P60775"
FT DISULFID 38..60
FT /evidence="ECO:0000250|UniProtKB:P60775"
FT DISULFID 62..73
FT /evidence="ECO:0000250|UniProtKB:P60775"
FT DISULFID 74..79
FT /evidence="ECO:0000250|UniProtKB:P60775"
FT CONFLICT 75
FT /note="T -> Q (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000269|PubMed:23438486"
SQ SEQUENCE 81 AA; 8980 MW; 43235EB2FB422736 CRC64;
MKTLLLTLVV VTIVCLDFGH TRICYNQQSS QPPTTKTCSE GQCYKKTWRD HRGTIIERGC
ACPNVKPGIQ ISCCTSDKCN G
