ID   ALR_PISSA               Reviewed;         166 AA.
AC   Q9F9L1;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Alanine racemase;
DE            EC=5.1.1.1;
DE   Flags: Fragment;
GN   Name=alr;
OS   Piscirickettsia salmonis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC   Piscirickettsiaceae; Piscirickettsia.
OX   NCBI_TaxID=1238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LF-89;
RA   Kuzyk M.A., Burian J., Thornton J.C., Kay W.W.;
RT   "Identification of a genus-common Rickettsial surface antigen in the
RT   salmonid pathogen Piscirickettsia salmonis.";
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the interconversion of L-alanine and D-alanine. May
CC       also act on other amino acids (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-alanine = D-alanine; Xref=Rhea:RHEA:20249,
CC         ChEBI:CHEBI:57416, ChEBI:CHEBI:57972; EC=5.1.1.1;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- PATHWAY: Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine
CC       from L-alanine: step 1/1.
CC   -!- SIMILARITY: Belongs to the alanine racemase family. {ECO:0000305}.
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DR   EMBL; AF184152; AAG17001.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9F9L1; -.
DR   SMR; Q9F9L1; -.
DR   STRING; 1238.AWJ11_09965; -.
DR   UniPathway; UPA00042; UER00497.
DR   GO; GO:0008784; F:alanine racemase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030632; P:D-alanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.40.37.10; -; 1.
DR   Gene3D; 3.20.20.10; -; 1.
DR   InterPro; IPR000821; Ala_racemase.
DR   InterPro; IPR009006; Ala_racemase/Decarboxylase_C.
DR   InterPro; IPR011079; Ala_racemase_C.
DR   InterPro; IPR001608; Ala_racemase_N.
DR   InterPro; IPR029066; PLP-binding_barrel.
DR   Pfam; PF00842; Ala_racemase_C; 1.
DR   Pfam; PF01168; Ala_racemase_N; 1.
DR   PRINTS; PR00992; ALARACEMASE.
DR   SMART; SM01005; Ala_racemase_C; 1.
DR   SUPFAM; SSF50621; SSF50621; 1.
DR   SUPFAM; SSF51419; SSF51419; 1.
DR   TIGRFAMs; TIGR00492; alr; 1.
PE   3: Inferred from homology;
KW   Isomerase; Pyridoxal phosphate.
FT   CHAIN           <1..166
FT                   /note="Alanine racemase"
FT                   /id="PRO_0000114545"
FT   ACT_SITE        62
FT                   /note="Proton acceptor; specific for L-alanine"
FT                   /evidence="ECO:0000250"
FT   BINDING         110
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   166 AA;  18409 MW;  E9C7B0B08FC2FE0D CRC64;
     NSAAIFNFSY ERYDWVRPGI MLYGISPFAD KNGVDLELQP VMHVVSRLIS VKQLRQGESV
     GYGATWQCPE DMQVGILSLG YGDGYPRLAA SGTPFLVRGQ RCALIGRVSM DMIAIDLRRC
     PDAGVGEAVT VWGQDLPVEE IARHVGTIAY ELVCNMPLRA PYIWQE