3S11_MICNI
ID 3S11_MICNI Reviewed; 60 AA.
AC P80548; Q9PRQ5;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Three-finger toxin Mnn I {ECO:0000303|PubMed:8665942};
DE AltName: Full=Neurotoxin alpha;
DE AltName: Full=Nicotinic acetylcholine receptor-binding protein Mnn-9;
DE AltName: Full=Short neurotoxin 1;
OS Micrurus nigrocinctus (Central American coral snake) (Gargantilla).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Micrurus.
OX NCBI_TaxID=8635;
RN [1]
RP PROTEIN SEQUENCE, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=8665942; DOI=10.1111/j.1432-1033.1996.0231q.x;
RA Rosso J.-P., Vargas-Rosso O., Gutierrez J.-M., Rochat H., Bougis P.E.;
RT "Characterization of alpha-neurotoxin and phospholipase A2 activities from
RT Micrurus venoms. Determination of the amino acid sequence and receptor-
RT binding ability of the major alpha-neurotoxin from Micrurus nigrocinctus
RT nigrocinctus.";
RL Eur. J. Biochem. 238:231-239(1996).
RN [2]
RP PROTEIN SEQUENCE OF 1-26.
RC TISSUE=Venom;
RX PubMed=8603741; DOI=10.1016/0014-5793(95)01543-4;
RA Alape-Giron A., Persson B., Cedelund E., Flores-Diaz M., Gutierrez J.-M.,
RA Thelestam M., Bergman T., Joernvall H.;
RT "Characterization of multiple nicotinic acetylcholine receptor-binding
RT proteins and phospholipases A2 from the venom of the coral snake Micrurus
RT nigrocinctus.";
RL FEBS Lett. 380:29-32(1996).
CC -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC inhibit acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular transmission. {ECO:0000250|UniProtKB:P60775}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8665942}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- TOXIC DOSE: LD(50) is 65 ug/kg by intraperitoneal injection into mice
CC (1.3 ug/mouse). {ECO:0000269|PubMed:8665942}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR PIR; S68641; S68641.
DR PIR; S68769; S68769.
DR AlphaFoldDB; P80548; -.
DR SMR; P80548; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Toxin.
FT CHAIN 1..60
FT /note="Three-finger toxin Mnn I"
FT /evidence="ECO:0000269|PubMed:8665942"
FT /id="PRO_0000093590"
FT DISULFID 3..22
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 17..39
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 41..52
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 53..58
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT CONFLICT 14
FT /note="I -> T (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 60 AA; 6583 MW; 4E9580F886F0279C CRC64;
MICHNQQSSQ PPTIKTCSEG QCYKKTWRDH RGTISERGCG CPTVKPGIHI SCCASDKCNA
