ID   ALR_PSETA               Reviewed;         357 AA.
AC   B9WZ64;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Alanine racemase {ECO:0000255|HAMAP-Rule:MF_01201};
DE            EC=5.1.1.1 {ECO:0000255|HAMAP-Rule:MF_01201};
GN   Name=alr {ECO:0000303|PubMed:19300994};
OS   Pseudomonas taetrolens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=47884;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE
RP   SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   STRAIN=ATCC 4683 / DSM 21104 / JCM 20238 / CCUG 560 / NBRC 3460 / NCIMB
RC   9396 / NCTC 10697 / NRRL B-14;
RX   PubMed=19300994; DOI=10.1007/s00253-009-1942-7;
RA   Matsui D., Oikawa T., Arakawa N., Osumi S., Lausberg F., Stabler N.,
RA   Freudl R., Eggeling L.;
RT   "A periplasmic, pyridoxal-5'-phosphate-dependent amino acid racemase in
RT   Pseudomonas taetrolens.";
RL   Appl. Microbiol. Biotechnol. 83:1045-1054(2009).
CC   -!- FUNCTION: Catalyzes the interconversion of L-alanine and D-alanine. Is
CC       highly specific for alanine as substrate. May serve both anabolic and
CC       catabolic purposes. {ECO:0000269|PubMed:19300994}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-alanine = D-alanine; Xref=Rhea:RHEA:20249,
CC         ChEBI:CHEBI:57416, ChEBI:CHEBI:57972; EC=5.1.1.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01201,
CC         ECO:0000269|PubMed:19300994};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01201};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.0. {ECO:0000269|PubMed:19300994};
CC       Temperature dependence:
CC         Optimum temperature is 35 degrees Celsius.
CC         {ECO:0000269|PubMed:19300994};
CC   -!- PATHWAY: Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine
CC       from L-alanine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_01201}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19300994}.
CC   -!- SIMILARITY: Belongs to the alanine racemase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01201}.
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DR   EMBL; AB296103; BAH23434.1; -; Genomic_DNA.
DR   AlphaFoldDB; B9WZ64; -.
DR   SMR; B9WZ64; -.
DR   UniPathway; UPA00042; UER00497.
DR   GO; GO:0008784; F:alanine racemase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0030632; P:D-alanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.40.37.10; -; 1.
DR   Gene3D; 3.20.20.10; -; 1.
DR   HAMAP; MF_01201; Ala_racemase; 1.
DR   InterPro; IPR000821; Ala_racemase.
DR   InterPro; IPR009006; Ala_racemase/Decarboxylase_C.
DR   InterPro; IPR011079; Ala_racemase_C.
DR   InterPro; IPR001608; Ala_racemase_N.
DR   InterPro; IPR020622; Ala_racemase_pyridoxalP-BS.
DR   InterPro; IPR029066; PLP-binding_barrel.
DR   Pfam; PF00842; Ala_racemase_C; 1.
DR   Pfam; PF01168; Ala_racemase_N; 1.
DR   PRINTS; PR00992; ALARACEMASE.
DR   SMART; SM01005; Ala_racemase_C; 1.
DR   SUPFAM; SSF50621; SSF50621; 1.
DR   SUPFAM; SSF51419; SSF51419; 1.
DR   TIGRFAMs; TIGR00492; alr; 1.
DR   PROSITE; PS00395; ALANINE_RACEMASE; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Pyridoxal phosphate.
FT   CHAIN           1..357
FT                   /note="Alanine racemase"
FT                   /id="PRO_0000419112"
FT   ACT_SITE        33
FT                   /note="Proton acceptor; specific for D-alanine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT   ACT_SITE        253
FT                   /note="Proton acceptor; specific for L-alanine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT   BINDING         129
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT   BINDING         301
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT   MOD_RES         33
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
SQ   SEQUENCE   357 AA;  38814 MW;  9CD988FF18E944DA CRC64;
     MRPARALIDL QALRHNYRLA RELTGAKALA VIKADAYGHG AVRCALALEA EADGFAVACI
     EEALELRAAG IKAPVLLLEG FFEASELALI AEHDLWCVVH SLWQLEAIER TQLHKPLTVW
     LKLDSGMHRV GLHPKDYHEA YQRLLASGKV ARIVLMSHFA RADELDADAT AQQIAVFEAA
     RQGLAAECSL RNSPGVLGWP QAPSDWVRPG LMLYGATPFE VAQAEAERLQ PVMTLQSRVI
     SVRELPAGEP VGYGAKFVSP RPTRVGVVAM GYADGYPRQA PNGTPVLVAG KRTQLIGRVS
     MDMLSIDLTD VPEATVGSPV ELWGKHVLAS EVAAHAGTIP YQIFCNLKRV PRDYIGE