GLGS_ECOLI
ID GLGS_ECOLI Reviewed; 66 AA.
AC P26649;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Surface composition regulator {ECO:0000255|HAMAP-Rule:MF_00525, ECO:0000303|PubMed:23537328};
GN Name=glgS {ECO:0000303|PubMed:1324388};
GN Synonyms=scoR {ECO:0000303|PubMed:23537328};
GN OrderedLocusNames=b3049, JW3021;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=K12;
RX PubMed=1324388; DOI=10.1111/j.1365-2958.1992.tb01360.x;
RA Hengge-Aronis R., Fischer D.;
RT "Identification and molecular analysis of glgS, a novel growth-phase-
RT regulated and rpoS-dependent gene involved in glycogen synthesis in
RT Escherichia coli.";
RL Mol. Microbiol. 6:1877-1886(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / BW25113;
RX PubMed=23537328; DOI=10.1042/bj20130154;
RA Rahimpour M., Montero M., Almagro G., Viale A.M., Sevilla A., Canovas M.,
RA Munoz F.J., Baroja-Fernandez E., Bahaji A., Eydallin G., Dose H.,
RA Takeuchi R., Mori H., Pozueta-Romero J.;
RT "GlgS, described previously as a glycogen synthesis control protein,
RT negatively regulates motility and biofilm formation in Escherichia coli.";
RL Biochem. J. 452:559-573(2013).
RN [6] {ECO:0007744|PDB:1RRZ}
RP STRUCTURE BY NMR.
RX PubMed=9208918; DOI=10.1111/j.1432-1033.1997.t01-1-00301.x;
RA Beglova N., Fischer D., Hengge-Aronis R., Gehring K.;
RT "1H, 15N and 13C NMR assignments, secondary structure and overall topology
RT of the Escherichia coli GlgS protein.";
RL Eur. J. Biochem. 246:301-310(1997).
CC -!- FUNCTION: Major determinant of cell surface composition. Negatively
CC regulates motility, adhesion and synthesis of biofilm
CC exopolysaccharides. {ECO:0000255|HAMAP-Rule:MF_00525,
CC ECO:0000269|PubMed:23537328}.
CC -!- INDUCTION: Expression is dependent on RpoS.
CC {ECO:0000269|PubMed:1324388}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutants show increased flagella and
CC fimbriae production, are hypermotile, and exhibit increased biofilm
CC formation. {ECO:0000269|PubMed:23537328}.
CC -!- SIMILARITY: Belongs to the GlgS family. {ECO:0000255|HAMAP-
CC Rule:MF_00525}.
CC -!- CAUTION: Was originally thought to be involved in glycogen synthesis,
CC but it was shown later that its effect on glycogen metabolism is
CC probably indirect. {ECO:0000305|PubMed:1324388,
CC ECO:0000305|PubMed:23537328}.
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DR EMBL; Z11885; CAA77940.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76085.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16577.1; -; Genomic_DNA.
DR PIR; S25201; S25201.
DR RefSeq; NP_417521.1; NC_000913.3.
DR RefSeq; WP_000350095.1; NZ_STEB01000001.1.
DR PDB; 1RRZ; NMR; -; A=1-66.
DR PDBsum; 1RRZ; -.
DR AlphaFoldDB; P26649; -.
DR SMR; P26649; -.
DR BioGRID; 4262385; 14.
DR IntAct; P26649; 2.
DR STRING; 511145.b3049; -.
DR jPOST; P26649; -.
DR PaxDb; P26649; -.
DR PRIDE; P26649; -.
DR EnsemblBacteria; AAC76085; AAC76085; b3049.
DR EnsemblBacteria; BAA16577; BAA16577; BAA16577.
DR GeneID; 66673054; -.
DR GeneID; 947533; -.
DR KEGG; ecj:JW3021; -.
DR KEGG; eco:b3049; -.
DR PATRIC; fig|1411691.4.peg.3682; -.
DR EchoBASE; EB1355; -.
DR eggNOG; ENOG5032ZQX; Bacteria.
DR HOGENOM; CLU_185971_0_0_6; -.
DR OMA; HRSWFCK; -.
DR PhylomeDB; P26649; -.
DR BioCyc; EcoCyc:EG11381-MON; -.
DR EvolutionaryTrace; P26649; -.
DR PRO; PR:P26649; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1902201; P:negative regulation of bacterial-type flagellum-dependent cell motility; IMP:EcoCyc.
DR GO; GO:1900232; P:negative regulation of single-species biofilm formation on inanimate substrate; IMP:EcoCyc.
DR GO; GO:0010676; P:positive regulation of cellular carbohydrate metabolic process; IMP:EcoCyc.
DR Gene3D; 1.20.970.20; -; 1.
DR HAMAP; MF_00525; GlgS; 1.
DR InterPro; IPR015065; GlgS.
DR InterPro; IPR036295; GlgS_sf.
DR Pfam; PF08971; GlgS; 1.
DR SUPFAM; SSF109747; SSF109747; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..66
FT /note="Surface composition regulator"
FT /id="PRO_0000071599"
FT TURN 3..6
FT /evidence="ECO:0007829|PDB:1RRZ"
FT HELIX 7..24
FT /evidence="ECO:0007829|PDB:1RRZ"
FT HELIX 30..36
FT /evidence="ECO:0007829|PDB:1RRZ"
FT HELIX 41..59
FT /evidence="ECO:0007829|PDB:1RRZ"
SQ SEQUENCE 66 AA; 7892 MW; D047AB6E7AD67523 CRC64;
MDHSLNSLNN FDFLARSFAR MHAEGRPVDI LAVTGNMDEE HRTWFCARYA WYCQQMMQAR
ELELEH
