ID   GLGX_HAEIN              Reviewed;         659 AA.
AC   P45178;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Glycogen operon protein GlgX homolog;
DE            EC=3.2.1.-;
GN   Name=glgX; OrderedLocusNames=HI_1358;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
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DR   EMBL; L42023; AAC23005.1; -; Genomic_DNA.
DR   PIR; A64119; A64119.
DR   RefSeq; NP_439509.1; NC_000907.1.
DR   RefSeq; WP_005694002.1; NC_000907.1.
DR   AlphaFoldDB; P45178; -.
DR   SMR; P45178; -.
DR   STRING; 71421.HI_1358; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   PRIDE; P45178; -.
DR   DNASU; 949470; -.
DR   EnsemblBacteria; AAC23005; AAC23005; HI_1358.
DR   KEGG; hin:HI_1358; -.
DR   PATRIC; fig|71421.8.peg.1411; -.
DR   eggNOG; COG1523; Bacteria.
DR   HOGENOM; CLU_011725_1_1_6; -.
DR   OMA; SEPWDCG; -.
DR   PhylomeDB; P45178; -.
DR   BioCyc; HINF71421:G1GJ1-1383-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   InterPro; IPR040784; GlgX_C.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF18390; GlgX_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR02100; glgX_debranch; 1.
PE   3: Inferred from homology;
KW   Glycogen biosynthesis; Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..659
FT                   /note="Glycogen operon protein GlgX homolog"
FT                   /id="PRO_0000054305"
FT   REGION          456..486
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        457..471
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        333
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        369
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   SITE            441
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   659 AA;  75290 MW;  E2B71F610E8B1CAC CRC64;
     MFKIYNNGNP IPMGYSQAVE NNVQITNFAL FSAAAIGVEL CLFDEQNQET RLPMVRTENV
     WHLAVTGVKT GTEYAFRIHG EFANPQKLIL DPYAKAVNGK PDLSSEESRS WFLLSDNRDN
     AHLAPRAVVI SEEFDWENDT SPNTPWAETI VYELHVKGFS QLNEKIPAAL RGTYTGLAHP
     VNLAYLKELG VTAVELLPVN FHINEPHLQA RGLQNYWGYN PLAMFAVEPK YAATNNPLAE
     FKTMVKAFHK AGIEVILDVV FNHSAESEQT YPTFSQRGID DQTYYWRNDQ GRYINWTGCG
     NMLNLSSDVG RKWVVDCLRY WVEQCHIDGF RFDLATVLGR DTPDFNSSAQ LFTDIKNEPS
     LQNIKLIAEP WDIGHYGYQV GNFPSYFAEW NDRFRDDLCR FWLWKSGEIG AFAERFAGSS
     DLFKKNDRLP HTTLNFITAH DGFTLKDLVS YNQKHNETNG EENRDGRNEN YSYNHGVEGS
     TESLSEPQKS AVENNRTFAQ SGLLMSLLLA NGTPMLLAGD EFGNTQYGNN NAYCQDNEIT
     WLKWANFNEE LFELTKQTIA LRKQIGSLNK DQWWSDENVQ WLNIVGEPMT VEDWQNQQTK
     ALQVVLDNRW LLLINAKAEG QMFHLPNRKW KPQIGTHNVT LEAQQAELSS MGFCMLNDE