ID   GLGX_MYCBO              Reviewed;         721 AA.
AC   P0A4Y5; A0A1R3XZL8; Q10767; X2BI83;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Glycogen operon protein GlgX homolog;
DE            EC=3.2.1.-;
GN   Name=glgX; Synonyms=treX; OrderedLocusNames=BQ2027_MB1591C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00194.1; -; Genomic_DNA.
DR   RefSeq; NP_855243.1; NC_002945.3.
DR   RefSeq; WP_003898930.1; NC_002945.4.
DR   AlphaFoldDB; P0A4Y5; -.
DR   SMR; P0A4Y5; -.
DR   PRIDE; P0A4Y5; -.
DR   EnsemblBacteria; SIU00194; SIU00194; BQ2027_MB1591C.
DR   PATRIC; fig|233413.5.peg.1738; -.
DR   OMA; SEPWDCG; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR02100; glgX_debranch; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..721
FT                   /note="Glycogen operon protein GlgX homolog"
FT                   /id="PRO_0000054306"
FT   ACT_SITE        362
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        398
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   SITE            470
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   721 AA;  81081 MW;  5616457CDCC887D1 CRC64;
     MSSNNAGESD GTGPALPTVW PGNAYPLGAT YDGAGTNFSL FSEIAEKVEL CLIDEDGVES
     RIPLDEVDGY VWHAYLPNIT PGQRYGFRVH GPFDPAAGHR CDPSKLLLDP YGKSFHGDFT
     FGQALYSYDV NAVDPDSTPP MVDSLGHTMT SVVINPFFDW AYDRSPRTPY HETVIYEAHV
     KGMTQTHPSI PPELRGTYAG LAHPVIIDHL NELNVTAVEL MPVHQFLHDS RLLDLGLRNY
     WGYNTFGFFA PHHQYASTRQ AGSAVAEFKT MVRSLHEAGI EVILDVVYNH TAEGNHLGPT
     INFRGIDNTA YYRLMDHDLR FYKDFTGTGN SLNARHPHTL QLIMDSLRYW VIEMHVDGFR
     FDLASTLARE LHDVDRLSAF FDLVQQDPVV SQVKLIAEPW DVGEGGYQVG NFPGLWTEWN
     GKYRDTVRDY WRGEPATLGE FASRLTGSSD LYEATGRRPS ASINFVTAHD GFTLNDLVSY
     NDKHNEANGE NNRDGESYNR SWNCGVEGPT DDPDILALRA RQMRNMWATL MVSQGTPMIA
     HGDEIGRTQY GNNNVYCQDS ELSWMDWSLV DKNADLLAFA RKATTLRKNH KVFRRRRFFE
     GEPIRSGDEV RDIAWLTPSG REMTHEDWGR GFDRCVAVFL NGEAITAPDA RGERVVDDSF
     LLCFNAHDHD VEFVMPHDGY AQQWTGELDT NDPVGDIDLT VTATDTFSVP ARSLLVLRKT
     L