GLI4_HUMAN
ID GLI4_HUMAN Reviewed; 376 AA.
AC P10075; Q96CK9;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 193.
DE RecName: Full=Zinc finger protein GLI4;
DE AltName: Full=Krueppel-related zinc finger protein 4;
DE AltName: Full=Protein HKR4;
GN Name=GLI4; Synonyms=HKR4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-272 AND 283-376, AND VARIANT
RP THR-180.
RX PubMed=2850480; DOI=10.1128/mcb.8.8.3104-3113.1988;
RA Ruppert J.M., Kinzler K.W., Wong A.J., Bigner S.H., Kao F.T., Law M.L.,
RA Seuanez H.N., O'Brien S.J., Vogelstein B.;
RT "The GLI-Kruppel family of human genes.";
RL Mol. Cell. Biol. 8:3104-3113(1988).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19369195; DOI=10.1074/mcp.m800588-mcp200;
RA Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,
RA Mann M., Daub H.;
RT "Large-scale proteomics analysis of the human kinome.";
RL Mol. Cell. Proteomics 8:1751-1764(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- INTERACTION:
CC P10075; P28799: GRN; NbExp=3; IntAct=EBI-14061927, EBI-747754;
CC P10075; O60333-2: KIF1B; NbExp=3; IntAct=EBI-14061927, EBI-10975473;
CC P10075; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-14061927, EBI-5235340;
CC P10075; O76024: WFS1; NbExp=3; IntAct=EBI-14061927, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; BC014165; AAH14165.1; -; mRNA.
DR EMBL; M20678; AAA35990.1; -; Genomic_DNA.
DR EMBL; M20679; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS6398.1; -.
DR PIR; F31201; F31201.
DR RefSeq; NP_612474.1; NM_138465.3.
DR AlphaFoldDB; P10075; -.
DR SMR; P10075; -.
DR BioGRID; 109000; 152.
DR IntAct; P10075; 9.
DR STRING; 9606.ENSP00000345024; -.
DR iPTMnet; P10075; -.
DR PhosphoSitePlus; P10075; -.
DR BioMuta; GLI4; -.
DR DMDM; 33302619; -.
DR EPD; P10075; -.
DR jPOST; P10075; -.
DR MassIVE; P10075; -.
DR MaxQB; P10075; -.
DR PaxDb; P10075; -.
DR PeptideAtlas; P10075; -.
DR PRIDE; P10075; -.
DR ProteomicsDB; 52562; -.
DR Antibodypedia; 43401; 202 antibodies from 23 providers.
DR DNASU; 2738; -.
DR Ensembl; ENST00000340042.2; ENSP00000345024.1; ENSG00000250571.7.
DR Ensembl; ENST00000523522.5; ENSP00000430987.1; ENSG00000250571.7.
DR GeneID; 2738; -.
DR KEGG; hsa:2738; -.
DR MANE-Select; ENST00000340042.2; ENSP00000345024.1; NM_138465.4; NP_612474.1.
DR UCSC; uc003yxx.4; human.
DR CTD; 2738; -.
DR DisGeNET; 2738; -.
DR GeneCards; GLI4; -.
DR HGNC; HGNC:4320; GLI4.
DR HPA; ENSG00000250571; Low tissue specificity.
DR MIM; 165280; gene.
DR neXtProt; NX_P10075; -.
DR OpenTargets; ENSG00000250571; -.
DR PharmGKB; PA28723; -.
DR VEuPathDB; HostDB:ENSG00000250571; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163360; -.
DR HOGENOM; CLU_002678_1_0_1; -.
DR InParanoid; P10075; -.
DR OMA; QRIHYRE; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; P10075; -.
DR TreeFam; TF337438; -.
DR PathwayCommons; P10075; -.
DR SignaLink; P10075; -.
DR BioGRID-ORCS; 2738; 26 hits in 1099 CRISPR screens.
DR ChiTaRS; GLI4; human.
DR GenomeRNAi; 2738; -.
DR Pharos; P10075; Tbio.
DR PRO; PR:P10075; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; P10075; protein.
DR Bgee; ENSG00000250571; Expressed in lower esophagus mucosa and 96 other tissues.
DR ExpressionAtlas; P10075; baseline and differential.
DR Genevisible; P10075; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 6.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 7.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 7.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Zinc; Zinc-finger.
FT CHAIN 1..376
FT /note="Zinc finger protein GLI4"
FT /id="PRO_0000047267"
FT ZN_FING 183..205
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 211..233
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 239..261
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 267..289
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 295..317
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 323..345
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 351..373
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..76
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 86
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19369195,
FT ECO:0007744|PubMed:23186163"
FT VARIANT 180
FT /note="A -> T (in dbSNP:rs1056148)"
FT /evidence="ECO:0000269|PubMed:2850480"
FT /id="VAR_052734"
FT CONFLICT 144..146
FT /note="RVT -> ARD (in Ref. 2; AAA35990)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 41145 MW; 68B1A638CC0758BC CRC64;
MAALGDIQES PSVPSPVSLS SPGTPGTQHH EPQLHLHGHQ HGSPGSSPKV LSQPSDLDLQ
DVEEVEIGRD TFWPDSEPKP EQAPRSPGSQ APDEGAGGAL RSLLRSLPRR ARCSAGFGPE
SSAERPAGQP PGAVPCAQPR GAWRVTLVQQ AAAGPEGAPE RAAELGVNFG RSRQGSARGA
KPHRCEACGK SFKYNSLLLK HQRIHTGEKP YACHECGKRF RGWSGFIQHH RIHTGEKPYE
CGQCGRAFSH SSHFTQHLRI HNGEKPYKCG ECGQAFSQSS NLVRHQRLHT GEKPYACSQC
GKAFIWSSVL IEHQRIHTGE KPYECSDCGK AFRGRSHFFR HLRTHTGEKP FACGACGKAF
GQSSQLIQHQ RVHYRE
