GLIP1_ARATH
ID GLIP1_ARATH Reviewed; 374 AA.
AC Q9FLN0;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=GDSL esterase/lipase 1 {ECO:0000303|PubMed:16126835};
DE EC=3.1.1.- {ECO:0000305};
DE AltName: Full=Extracellular lipase 1;
DE Flags: Precursor;
GN Name=GLIP1 {ECO:0000303|PubMed:16126835};
GN OrderedLocusNames=At5g40990 {ECO:0000312|Araport:AT5G40990};
GN ORFNames=MEE6.6 {ECO:0000312|EMBL:BAB09701.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [4]
RP GENE FAMILY, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, DISRUPTION
RP PHENOTYPE, SUBCELLULAR LOCATION, AND INDUCTION BY ETHYLENE.
RX PubMed=16126835; DOI=10.1105/tpc.105.034819;
RA Oh I.S., Park A.R., Bae M.S., Kwon S.J., Kim Y.S., Lee J.E., Kang N.Y.,
RA Lee S., Cheong H., Park O.K.;
RT "Secretome analysis reveals an Arabidopsis lipase involved in defense
RT against Alternaria brassicicola.";
RL Plant Cell 17:2832-2847(2005).
RN [5]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- FUNCTION: Confers resistance to the necrotrophic fungus Alternaria
CC brassicicola. Possesses lipase and antimicrobial activities that
CC directly disrupt fungal spore integrity. Triggers systemic resistance,
CC mostly by the ethylene-dependent pathway.
CC {ECO:0000269|PubMed:16126835}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16126835}.
CC Note=Secreted upon salicylic acid treatment.
CC {ECO:0000269|PubMed:16126835}.
CC -!- INDUCTION: By ethylene. {ECO:0000269|PubMed:16126835}.
CC -!- DISRUPTION PHENOTYPE: In the resistant cv. Columbia (glip1-1 and glip1-
CC 2), confers susceptibility to A.brassicicola.
CC {ECO:0000269|PubMed:16126835}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AB010072; BAB09701.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94623.1; -; Genomic_DNA.
DR RefSeq; NP_198915.1; NM_123464.2.
DR AlphaFoldDB; Q9FLN0; -.
DR SMR; Q9FLN0; -.
DR STRING; 3702.AT5G40990.1; -.
DR PaxDb; Q9FLN0; -.
DR PRIDE; Q9FLN0; -.
DR ProteomicsDB; 230159; -.
DR EnsemblPlants; AT5G40990.1; AT5G40990.1; AT5G40990.
DR GeneID; 834101; -.
DR Gramene; AT5G40990.1; AT5G40990.1; AT5G40990.
DR KEGG; ath:AT5G40990; -.
DR Araport; AT5G40990; -.
DR TAIR; locus:2163021; AT5G40990.
DR eggNOG; ENOG502QQ4G; Eukaryota.
DR HOGENOM; CLU_015101_0_2_1; -.
DR InParanoid; Q9FLN0; -.
DR OMA; EASVACC; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q9FLN0; -.
DR BioCyc; ARA:AT5G40990-MON; -.
DR PRO; PR:Q9FLN0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLN0; baseline and differential.
DR Genevisible; Q9FLN0; AT.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0016298; F:lipase activity; IDA:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:0009866; P:induced systemic resistance, ethylene mediated signaling pathway; IMP:TAIR.
DR GO; GO:0009871; P:jasmonic acid and ethylene-dependent systemic resistance, ethylene mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009620; P:response to fungus; IMP:TAIR.
DR GO; GO:0009751; P:response to salicylic acid; IEP:TAIR.
DR GO; GO:0009627; P:systemic acquired resistance; IMP:UniProtKB.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR044552; GLIP1-5/GLL25.
DR InterPro; IPR008265; Lipase_GDSL_AS.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR PANTHER; PTHR45966; PTHR45966; 1.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE 1: Evidence at protein level;
KW Ethylene signaling pathway; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid metabolism; Plant defense; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..374
FT /note="GDSL esterase/lipase 1"
FT /id="PRO_0000367334"
FT ACT_SITE 44
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q09LX1"
FT ACT_SITE 338
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q09LX1"
FT ACT_SITE 341
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q09LX1"
FT CARBOHYD 34
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 184
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 203
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 330
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 360
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 374 AA; 41706 MW; 3CF3297DEB910456 CRC64;
MENSQLVSIT FLAYTIIISI GSINCIDNNN LVTNQSALFV FGDSVFDAGN NNYIDTLSSV
RSNYWPYGQT TFKSPTGRVS DGRLIPDFIA EYAWLPLIPP NLQPFNGNSQ FAYGVNFASG
GAGALVGTFS GLVINLRTQL NNFKKVEEML RSKLGDAEGK RVISRAVYLF HIGLNDYQYP
FTTNSSLFQS ISNEKYVDYV VGNMTDVFKE VYNLGGRKFG ILNTGPYDCA PASLVIDQTK
IRSCFQPVTE LINMHNEKLL NGLRRLNHEL SGFKYALHDY HTSLSERMND PSKYGFKEGK
KACCGSGPLR GINTCGGRMG LSQSYELCEN VTDYLFFDPF HLTEKANRQI AELIWSGPTN
ITGPYNLKAL FELN
