GLK1_ARATH
ID GLK1_ARATH Reviewed; 420 AA.
AC Q9SIV3; Q9C532;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Transcription activator GLK1;
DE AltName: Full=GBF'S PRO-RICH REGION-INTERACTING factor 1;
DE AltName: Full=Golden2-like protein 1;
DE Short=AtGLK1;
GN Name=GLK1; Synonyms=GPRI1; OrderedLocusNames=At2g20570; ORFNames=F23N11.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=11828027; DOI=10.1093/pcp/pcf011;
RA Tamai H., Iwabuchi M., Meshi T.;
RT "Arabidopsis GARP transcriptional activators interact with the Pro-rich
RT activation domain shared by G-box-binding bZIP factors.";
RL Plant Cell Physiol. 43:99-107(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY,
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12220263; DOI=10.1046/j.1365-313x.2002.01390.x;
RA Fitter D.W., Martin D.J., Copley M.J., Scotland R.W., Langdale J.A.;
RT "GLK gene pairs regulate chloroplast development in diverse plant
RT species.";
RL Plant J. 31:713-727(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION.
RX PubMed=17533111; DOI=10.1016/j.bbrc.2007.05.084;
RA Savitch L.V., Subramaniam R., Allard G.C., Singh J.;
RT "The GLK1 'regulon' encodes disease defense related proteins and confers
RT resistance to Fusarium graminearum in Arabidopsis.";
RL Biochem. Biophys. Res. Commun. 359:234-238(2007).
RN [7]
RP FUNCTION.
RX PubMed=18643989; DOI=10.1111/j.1365-313x.2008.03616.x;
RA Waters M.T., Moylan E.C., Langdale J.A.;
RT "GLK transcription factors regulate chloroplast development in a cell-
RT autonomous manner.";
RL Plant J. 56:432-444(2008).
RN [8]
RP FUNCTION.
RX PubMed=19383092; DOI=10.1111/j.1469-8137.2009.02829.x;
RA Bravo-Garcia A., Yasumura Y., Langdale J.A.;
RT "Specialization of the Golden2-like regulatory pathway during land plant
RT evolution.";
RL New Phytol. 183:133-141(2009).
RN [9]
RP FUNCTION.
RX PubMed=19376934; DOI=10.1105/tpc.108.065250;
RA Waters M.T., Wang P., Korkaric M., Capper R.G., Saunders N.J.,
RA Langdale J.A.;
RT "GLK transcription factors coordinate expression of the photosynthetic
RT apparatus in Arabidopsis.";
RL Plant Cell 21:1109-1128(2009).
RN [10]
RP FUNCTION.
RX PubMed=19726569; DOI=10.1104/pp.109.145987;
RA Kakizaki T., Matsumura H., Nakayama K., Che F.S., Terauchi R., Inaba T.;
RT "Coordination of plastid protein import and nuclear gene expression by
RT plastid-to-nucleus retrograde signaling.";
RL Plant Physiol. 151:1339-1353(2009).
RN [11]
RP INDUCTION.
RX PubMed=21214652; DOI=10.1111/j.1365-313x.2010.04449.x;
RA Yu X., Li L., Zola J., Aluru M., Ye H., Foudree A., Guo H., Anderson S.,
RA Aluru S., Liu P., Rodermel S., Yin Y.;
RT "A brassinosteroid transcriptional network revealed by genome-wide
RT identification of BESI target genes in Arabidopsis thaliana.";
RL Plant J. 65:634-646(2011).
RN [12]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH NAC92.
RC STRAIN=cv. Columbia;
RX PubMed=23459204; DOI=10.1038/embor.2013.24;
RA Rauf M., Arif M., Dortay H., Matallana-Ramirez L.P., Waters M.T.,
RA Gil Nam H., Lim P.-O., Mueller-Roeber B., Balazadeh S.;
RT "ORE1 balances leaf senescence against maintenance by antagonizing G2-like-
RT mediated transcription.";
RL EMBO Rep. 14:382-388(2013).
CC -!- FUNCTION: Transcriptional activator that functions with GLK2 to promote
CC chloroplast development. Acts as an activator of nuclear photosynthetic
CC genes involved in chlorophyll biosynthesis, light harvesting, and
CC electron transport. Acts in a cell-autonomous manner to coordinate and
CC maintain the photosynthetic apparatus within individual cells. May
CC function in photosynthetic capacity optimization by integrating
CC responses to variable environmental and endogenous cues
CC (PubMed:11828027, PubMed:12220263, PubMed:17533111, PubMed:18643989,
CC PubMed:19376934, PubMed:19383092, PubMed:19726569). Prevents premature
CC senescence (PubMed:23459204). {ECO:0000269|PubMed:11828027,
CC ECO:0000269|PubMed:12220263, ECO:0000269|PubMed:17533111,
CC ECO:0000269|PubMed:18643989, ECO:0000269|PubMed:19376934,
CC ECO:0000269|PubMed:19383092, ECO:0000269|PubMed:19726569,
CC ECO:0000269|PubMed:23459204}.
CC -!- SUBUNIT: Interacts with NAC92. {ECO:0000269|PubMed:23459204}.
CC -!- INTERACTION:
CC Q9SIV3; F4K1A8: At5g26749; NbExp=3; IntAct=EBI-4442165, EBI-15191587;
CC Q9SIV3; O82595: NGA4; NbExp=3; IntAct=EBI-4442165, EBI-15193945;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9FFH0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SIV3-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in rosette and cauline leaves. Expressed
CC at low levels in cotyledons and shoots. {ECO:0000269|PubMed:12220263}.
CC -!- INDUCTION: By light. Repressed by BZR2. {ECO:0000269|PubMed:12220263,
CC ECO:0000269|PubMed:21214652}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:12220263). Pale-
CC green seedlings in double mutants glk1/glk2 (PubMed:23459204).
CC {ECO:0000269|PubMed:12220263, ECO:0000269|PubMed:23459204}.
CC -!- MISCELLANEOUS: Plants overexpressing GLK1 have a delay in flowering
CC under long days, show high constitutive expression of genes encoding
CC disease defense related proteins and are resistant to the pathogen
CC F.graminearum.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB062489; BAB78466.1; -; mRNA.
DR EMBL; AY026772; AAK16743.1; -; mRNA.
DR EMBL; AY028367; AAK20120.1; -; Genomic_DNA.
DR EMBL; AC007048; AAD21709.2; -; Genomic_DNA.
DR EMBL; AC007109; AAM15392.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07030.1; -; Genomic_DNA.
DR EMBL; AY054600; AAK96791.1; -; mRNA.
DR EMBL; AY081485; AAM10047.1; -; mRNA.
DR PIR; H84590; H84590.
DR RefSeq; NP_565476.1; NM_127617.3. [Q9SIV3-1]
DR AlphaFoldDB; Q9SIV3; -.
DR SMR; Q9SIV3; -.
DR BioGRID; 1932; 59.
DR IntAct; Q9SIV3; 56.
DR MINT; Q9SIV3; -.
DR STRING; 3702.AT2G20570.2; -.
DR PaxDb; Q9SIV3; -.
DR PRIDE; Q9SIV3; -.
DR ProteomicsDB; 248449; -. [Q9SIV3-1]
DR EnsemblPlants; AT2G20570.1; AT2G20570.1; AT2G20570. [Q9SIV3-1]
DR GeneID; 816579; -.
DR Gramene; AT2G20570.1; AT2G20570.1; AT2G20570. [Q9SIV3-1]
DR KEGG; ath:AT2G20570; -.
DR Araport; AT2G20570; -.
DR eggNOG; ENOG502S88Y; Eukaryota.
DR HOGENOM; CLU_050767_0_0_1; -.
DR InParanoid; Q9SIV3; -.
DR OMA; ANVPPAC; -.
DR PhylomeDB; Q9SIV3; -.
DR PRO; PR:Q9SIV3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SIV3; baseline and differential.
DR Genevisible; Q9SIV3; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:1900056; P:negative regulation of leaf senescence; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR044825; GLK1/2-like.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR PANTHER; PTHR31312; PTHR31312; 1.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..420
FT /note="Transcription activator GLK1"
FT /id="PRO_0000408385"
FT DNA_BIND 150..209
FT /note="Myb-like GARP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 74..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..136
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 420 AA; 45988 MW; 6C5F1994F3FB836C CRC64;
MLALSPATRD GCDGASEFLD TSCGFTIINP EEEEEFPDFA DHGDLLDIID FDDIFGVAGD
VLPDLEIDPE ILSGDFSNHM NASSTITTTS DKTDSQGETT KGSSGKGEEV VSKRDDVAAE
TVTYDGDSDR KRKYSSSASS KNNRISNNEG KRKVKVDWTP ELHRRFVEAV EQLGVDKAVP
SRILELMGVH CLTRHNVASH LQKYRSHRKH LLAREAEAAN WTRKRHIYGV DTGANLNGRT
KNGWLAPAPT LGFPPPPPVA VAPPPVHHHH FRPLHVWGHP TVDQSIMPHV WPKHLPPPST
AMPNPPFWVS DSPYWHPMHN GTTPYLPTVA TRFRAPPVAG IPHALPPHHT MYKPNLGFGG
ARPPVDLHPS KESVDAAIGD VLTRPWLPLP LGLNPPAVDG VMTELHRHGV SEVPPTASCA
