ID   GLK_BURCA               Reviewed;         642 AA.
AC   Q1BYA7;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Bifunctional protein glk;
DE   Includes:
DE     RecName: Full=Glucokinase;
DE              EC=2.7.1.2;
DE     AltName: Full=Glucose kinase;
DE   Includes:
DE     RecName: Full=Putative HTH-type transcriptional regulator;
GN   Name=glk; OrderedLocusNames=Bcen_0486;
OS   Burkholderia cenocepacia (strain AU 1054).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=331271;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AU 1054;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K.,
RA   Tiedje J.M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.2;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the bacterial
CC       glucokinase family. {ECO:0000305}.
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DR   EMBL; CP000378; ABF75398.1; -; Genomic_DNA.
DR   RefSeq; WP_006476653.1; NC_008060.1.
DR   AlphaFoldDB; Q1BYA7; -.
DR   SMR; Q1BYA7; -.
DR   EnsemblBacteria; ABF75398; ABF75398; Bcen_0486.
DR   KEGG; bcn:Bcen_0486; -.
DR   HOGENOM; CLU_016801_0_0_4; -.
DR   OMA; WSHVSFE; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0004340; F:glucokinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniRule.
DR   CDD; cd05013; SIS_RpiR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_00524; Glucokinase; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR003836; Glucokinase.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR000281; HTH_RpiR.
DR   InterPro; IPR035472; RpiR-like_SIS.
DR   InterPro; IPR001347; SIS_dom.
DR   InterPro; IPR046348; SIS_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF02685; Glucokinase; 1.
DR   Pfam; PF01418; HTH_6; 1.
DR   Pfam; PF01380; SIS; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53067; SSF53067; 1.
DR   SUPFAM; SSF53697; SSF53697; 1.
DR   TIGRFAMs; TIGR00749; glk; 1.
DR   PROSITE; PS00356; HTH_LACI_1; 1.
DR   PROSITE; PS51071; HTH_RPIR; 1.
DR   PROSITE; PS51464; SIS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Glycolysis; Kinase; Membrane;
KW   Multifunctional enzyme; Nucleotide-binding; Transcription;
KW   Transcription regulation; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..642
FT                   /note="Bifunctional protein glk"
FT                   /id="PRO_0000268796"
FT   TRANSMEM        576..596
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          341..417
FT                   /note="HTH rpiR-type"
FT   DOMAIN          461..600
FT                   /note="SIS"
FT   DNA_BIND        377..396
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          1..340
FT                   /note="Glucokinase"
FT   REGION          341..642
FT                   /note="Putative HTH-type transcriptional regulator"
FT   BINDING         23..28
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   642 AA;  68521 MW;  3748356F462BC6B6 CRC64;
     MSTGAQSKAV VAGQHADGPR LLADVGGTNA RFALETGPGE ITQIRVYPGA DYPTITDAIR
     KYLKDVKISR VNHAAIAIAN PVDGDQVTMT NHDWSFSIEA TRRALGFDTL LVVNDFTALA
     MALPGLTDAQ RVQVGGGARR QNSVIGLLGP GTGLGVSGLI PADDRWIALG SEGGHASFAP
     QDEREDLVLQ YARKKFPHVS FERVCAGPGM EIIYRALAAR DKKRVAATVD TVEIVERAHA
     GDALALETVE CFCGILGAFA GSVALTLGAL GGVYIGGGVA LKLGELFTRS SFRARFEAKG
     RFTHYLENIP TYLITAEYPA FLGVSAILAE QLSNRSGGAS SAVFERIRQM RDALTPAERR
     VADLALNHPR SIINDPIVDI ARKADVSQPT VIRFCRSLGC QGLSDFKLKL ATGLTGTIPM
     SHSQVHLGDT ATDFGAKVLD NTVSAILQLR EHLNFEHVEN AIEILNGARR IEFYGLGNSN
     IVAQDAHYKF FRFGIPTIAY GDLYMQAASA ALLGKGDVIV AVSKSGRAPE LLRVLDVAMQ
     AGAKVIAITS SNTPLAKRAT VALETDHIEM RESQLSMISR ILHLLMIDIL AVGVAIRRAS
     TNGELPEAVA QAKARASDDE TADVLDWLSH GASPAAKDVA RD