GLK_ECO57
ID GLK_ECO57 Reviewed; 321 AA.
AC P0A6V9; P46880; P78276;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Glucokinase;
DE EC=2.7.1.2;
DE AltName: Full=Glucose kinase;
GN Name=glk; OrderedLocusNames=Z3654, ECs3268;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX PubMed=15466045; DOI=10.1128/jb.186.20.6915-6927.2004;
RA Lunin V.V., Li Y., Schrag J.D., Iannuzzi P., Cygler M., Matte A.;
RT "Crystal structures of Escherichia coli ATP-dependent glucokinase and its
RT complex with glucose.";
RL J. Bacteriol. 186:6915-6927(2004).
CC -!- FUNCTION: Not highly important in E.coli as glucose is transported into
CC the cell by the PTS system already as glucose 6-phosphate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.2;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bacterial glucokinase family. {ECO:0000305}.
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DR EMBL; AE005174; AAG57514.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB36691.1; -; Genomic_DNA.
DR PIR; D91037; D91037.
DR PIR; F85881; F85881.
DR RefSeq; NP_311295.1; NC_002695.1.
DR RefSeq; WP_000170346.1; NZ_SWKA01000005.1.
DR PDB; 1Q18; X-ray; 2.36 A; A/B=2-321.
DR PDB; 1SZ2; X-ray; 2.20 A; A/B=2-321.
DR PDBsum; 1Q18; -.
DR PDBsum; 1SZ2; -.
DR AlphaFoldDB; P0A6V9; -.
DR SMR; P0A6V9; -.
DR STRING; 155864.EDL933_3557; -.
DR EnsemblBacteria; AAG57514; AAG57514; Z3654.
DR EnsemblBacteria; BAB36691; BAB36691; ECs_3268.
DR GeneID; 915629; -.
DR KEGG; ece:Z3654; -.
DR KEGG; ecs:ECs_3268; -.
DR PATRIC; fig|386585.9.peg.3412; -.
DR eggNOG; COG0837; Bacteria.
DR HOGENOM; CLU_042582_1_0_6; -.
DR OMA; NNHWRLS; -.
DR BRENDA; 2.7.1.2; 2026.
DR EvolutionaryTrace; P0A6V9; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004340; F:glucokinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00524; Glucokinase; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR003836; Glucokinase.
DR Pfam; PF02685; Glucokinase; 1.
DR SUPFAM; SSF53067; SSF53067; 1.
DR TIGRFAMs; TIGR00749; glk; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; Glycolysis; Kinase;
KW Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..321
FT /note="Glucokinase"
FT /id="PRO_0000215125"
FT BINDING 8..13
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT STRAND 4..11
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 14..21
FT /evidence="ECO:0007829|PDB:1SZ2"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 27..34
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 41..51
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 58..65
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 75..77
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 83..90
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 93..99
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 100..107
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 113..115
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 130..147
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 150..155
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 158..160
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 168..180
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 181..183
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 192..205
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 215..224
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 228..252
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 257..261
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 263..265
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 266..268
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 269..274
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 277..282
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 285..287
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 288..291
FT /evidence="ECO:0007829|PDB:1SZ2"
FT STRAND 296..299
FT /evidence="ECO:0007829|PDB:1SZ2"
FT HELIX 304..316
FT /evidence="ECO:0007829|PDB:1SZ2"
SQ SEQUENCE 321 AA; 34723 MW; 2D6CD956641A3823 CRC64;
MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC
IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG
GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE AIILEILRAE
IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI
MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV
HDNPGLLGSG AHLRQTLGHI L