ID   GLK_STRLI               Reviewed;         317 AA.
AC   P0A4E2; P40184;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Glucokinase;
DE            EC=2.7.1.2;
DE   AltName: Full=Glucose kinase;
GN   Name=glkA; Synonyms=glk;
OS   Streptomyces lividans.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1916;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=TK24;
RX   PubMed=11386347; DOI=10.1023/a:1010234916745;
RA   Mahr K., van Wezel G.P., Svensson C., Krengel U., Bibb M.J., Titgemeyer F.;
RT   "Glucose kinase of Streptomyces coelicolor A3(2): large-scale purification
RT   and biochemical analysis.";
RL   Antonie Van Leeuwenhoek 78:253-261(2000).
CC   -!- FUNCTION: Required for glucose repression of many different genes.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.2;
CC   -!- SUBUNIT: Homooligomer (possibly a homotetramer). Alternatively, it may
CC       form a heterotetramer of two glucokinase subunits with two ORF2 (AC
CC       P40182) proteins.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the ROK (NagC/XylR) family. {ECO:0000305}.
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DR   EMBL; AF228048; AAF42869.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A4E2; -.
DR   SMR; P0A4E2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004340; F:glucokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-KW.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000600; ROK.
DR   InterPro; IPR004654; ROK_glcA.
DR   PANTHER; PTHR18964; PTHR18964; 1.
DR   Pfam; PF00480; ROK; 1.
DR   SUPFAM; SSF53067; SSF53067; 1.
DR   TIGRFAMs; TIGR00744; ROK_glcA_fam; 1.
DR   PROSITE; PS01125; ROK; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding; Repressor;
KW   Transferase.
FT   CHAIN           1..317
FT                   /note="Glucokinase"
FT                   /id="PRO_0000095678"
SQ   SEQUENCE   317 AA;  33062 MW;  6E65A97691BA15BF CRC64;
     MGLTIGVDIG GTKIAAGVVD EEGNILSTHK VPTPTTPEAI VDAIASAVEG ARVGHEIVAV
     GIGAAGYVNR QRSTVYFAPN IDWRQEPLKE KVEARVGLPV VVENDANAAA WGEYKFGGGK
     GHRNVICITL GTGLGGGIII GNKLRRGHFG VAAEFGHIRM VPDGLLCGCG SQGCWEQYAS
     GRALVRYAKQ RANATPERAE VLLALGDGTP DGIEGKHISV AARQGCPVAV DSYRELARWA
     GAGLADLASL FDPSAFIVGG GLSDEGDLVL DPIRKSYKRW LVGGNWRPVA DVIAAQLGNK
     AGLVGAADLA REPDPIM