ALS3_ARATH
ID ALS3_ARATH Reviewed; 273 AA.
AC Q9ZUT3;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Protein ALUMINUM SENSITIVE 3;
DE AltName: Full=ABC transporter I family member 16;
DE Short=ABC transporter ABCI.16;
DE Short=AtABCI16;
DE AltName: Full=ybbM homolog protein;
GN Name=ALS3; Synonyms=ABCI16; OrderedLocusNames=At2g37330; ORFNames=F3G5.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF SER-112, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY ALUMINUM.
RX PubMed=15659095; DOI=10.1111/j.1365-313x.2004.02306.x;
RA Larsen P.B., Geisler M.J.B., Jones C.A., Williams K.M., Cancel J.D.;
RT "ALS3 encodes a phloem-localized ABC transporter-like protein that is
RT required for aluminum tolerance in Arabidopsis.";
RL Plant J. 41:353-363(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF SER-112.
RX PubMed=8819866; DOI=10.1104/pp.110.3.743;
RA Larsen P.B., Tai C.-Y., Kochian L.V., Howell S.H.;
RT "Arabidopsis mutants with increased sensitivity to aluminum.";
RL Plant Physiol. 110:743-751(1996).
RN [6]
RP FUNCTION, AND MUTAGENESIS OF SER-112.
RX PubMed=12223767; DOI=10.1104/pp.114.4.1207;
RA Larsen P.B., Kochian L.V., Howell S.H.;
RT "Al inhibits both shoot development and root growth in als3, an Al-
RT sensitive Arabidopsis mutant.";
RL Plant Physiol. 114:1207-1214(1997).
RN [7]
RP TRANSCRIPT MOBILITY.
RX PubMed=18485061; DOI=10.1111/j.1365-313x.2008.03555.x;
RA Deeken R., Ache P., Kajahn I., Klinkenberg J., Bringmann G., Hedrich R.;
RT "Identification of Arabidopsis thaliana phloem RNAs provides a search
RT criterion for phloem-based transcripts hidden in complex datasets of
RT microarray experiments.";
RL Plant J. 55:746-759(2008).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
RN [9]
RP INDUCTION BY STOP1.
RX PubMed=19321711; DOI=10.1104/pp.108.134700;
RA Sawaki Y., Iuchi S., Kobayashi Y., Kobayashi Y., Ikka T., Sakurai N.,
RA Fujita M., Shinozaki K., Shibata D., Kobayashi M., Koyama H.;
RT "STOP1 regulates multiple genes that protect arabidopsis from proton and
RT aluminum toxicities.";
RL Plant Physiol. 150:281-294(2009).
CC -!- FUNCTION: Required for aluminum (Al) resistance/tolerance, probably by
CC translocating Al from sensitive tissues such as growing roots to
CC tissues less sensisitive to the toxic effects of Al.
CC {ECO:0000269|PubMed:12223767, ECO:0000269|PubMed:15659095,
CC ECO:0000269|PubMed:8819866}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15659095};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15659095}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, and flowers.
CC {ECO:0000269|PubMed:15659095}.
CC -!- INDUCTION: By Al, primarily in leaf hydathodes and the phloem
CC throughout the plant, along with the root cortex. Regulated positively
CC by STOP1. {ECO:0000269|PubMed:15659095, ECO:0000269|PubMed:19321711}.
CC -!- MISCELLANEOUS: ALS3 transcripts are mobile in the phloem sap.
CC -!- SIMILARITY: Belongs to the UPF0014 family. {ECO:0000305}.
CC -!- CAUTION: Was originally (PubMed:18299247) thought to belong to the ABC
CC transporter family. Lacks the conserved ABC domain, which is one of the
CC features of the ABC transporter family. {ECO:0000305|PubMed:18299247}.
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DR EMBL; AY687991; AAV49319.1; -; mRNA.
DR EMBL; AC005896; AAC98053.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09384.1; -; Genomic_DNA.
DR EMBL; AY072359; AAL62351.1; -; mRNA.
DR EMBL; AY114725; AAM48044.1; -; mRNA.
DR PIR; D84791; D84791.
DR RefSeq; NP_181270.1; NM_129289.5.
DR AlphaFoldDB; Q9ZUT3; -.
DR BioGRID; 3654; 35.
DR IntAct; Q9ZUT3; 35.
DR STRING; 3702.AT2G37330.1; -.
DR TCDB; 3.A.1.139.4; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q9ZUT3; -.
DR PRIDE; Q9ZUT3; -.
DR ProteomicsDB; 244952; -.
DR EnsemblPlants; AT2G37330.1; AT2G37330.1; AT2G37330.
DR GeneID; 818310; -.
DR Gramene; AT2G37330.1; AT2G37330.1; AT2G37330.
DR KEGG; ath:AT2G37330; -.
DR Araport; AT2G37330; -.
DR TAIR; locus:2049751; AT2G37330.
DR eggNOG; ENOG502RHEJ; Eukaryota.
DR HOGENOM; CLU_076147_1_0_1; -.
DR InParanoid; Q9ZUT3; -.
DR OMA; PWYEPQY; -.
DR OrthoDB; 1036153at2759; -.
DR PhylomeDB; Q9ZUT3; -.
DR PRO; PR:Q9ZUT3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZUT3; baseline and differential.
DR Genevisible; Q9ZUT3; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0010044; P:response to aluminum ion; IMP:TAIR.
DR InterPro; IPR005226; UPF0014_fam.
DR PANTHER; PTHR30028; PTHR30028; 1.
DR Pfam; PF03649; UPF0014; 1.
DR TIGRFAMs; TIGR00245; TIGR00245; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..273
FT /note="Protein ALUMINUM SENSITIVE 3"
FT /id="PRO_0000379146"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 112
FT /note="S->L: In als3-1; inhibited leaf expansion and root
FT growth in the presence of Al."
FT /evidence="ECO:0000269|PubMed:12223767,
FT ECO:0000269|PubMed:15659095, ECO:0000269|PubMed:8819866"
SQ SEQUENCE 273 AA; 29887 MW; 4876387FDD2B2704 CRC64;
MDLKWDDFFN DYEWLIVFLK GMVKPAAALV VVLLAVILSY SQNLSLEGEM IYSVSRSFLQ
LSVIGFVLQF IFNQENSGWI ILAYLFMVSV AGYTAGQRAR HVPRGKYVAG LSILAGTSIT
MFLLVLLNVF PFTPRYMIPI AGMLVGNAMT VTGVTMKQLR DDIKMQLNLV ETALALGATP
RQATLQQVKR ALVISLSPVL DSCKTVGLIS LPGAMTGMIM GGASPLEAIQ LQIVVMNMMV
GAATVSSITS TYLCWPSFFT KAYQLQTHVF SSD
