ID   ALSA_ECOLI              Reviewed;         510 AA.
AC   P32721; Q2M6L6;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   03-AUG-2022, entry version 167.
DE   RecName: Full=D-allose import ATP-binding protein AlsA;
DE            EC=7.5.2.8 {ECO:0000305|PubMed:9401019};
GN   Name=alsA; Synonyms=yjcW; OrderedLocusNames=b4087, JW4048;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 9-510.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA   Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT   "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT   from 89.2 to 92.8 minutes.";
RL   Nucleic Acids Res. 21:5408-5417(1993).
RN   [5]
RP   FUNCTION.
RX   PubMed=9401019; DOI=10.1128/jb.179.24.7631-7637.1997;
RA   Kim C., Song S., Park C.;
RT   "The D-allose operon of Escherichia coli K-12.";
RL   J. Bacteriol. 179:7631-7637(1997).
CC   -!- FUNCTION: Part of the ABC transporter complex AlsBAC involved in D-
CC       allose import. Probably responsible for energy coupling to the
CC       transport system. {ECO:0000269|PubMed:9401019}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-allose(out) + H2O = ADP + D-allose(in) + H(+) +
CC         phosphate; Xref=Rhea:RHEA:29799, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:40822,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.5.2.8;
CC         Evidence={ECO:0000305|PubMed:9401019};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (AlsA),
CC       two transmembrane proteins (AlsC) and a solute-binding protein (AlsB).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. D-allose
CC       importer (TC 3.A.1.2.6) family. {ECO:0000305}.
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DR   EMBL; U14003; AAA96986.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77048.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78090.1; -; Genomic_DNA.
DR   EMBL; U00006; AAC43181.1; -; Genomic_DNA.
DR   PIR; S56315; S56315.
DR   RefSeq; NP_418511.1; NC_000913.3.
DR   RefSeq; WP_000235257.1; NZ_SSZK01000016.1.
DR   AlphaFoldDB; P32721; -.
DR   SMR; P32721; -.
DR   BioGRID; 4260960; 6.
DR   ComplexPortal; CPX-4320; D-allose ABC transporter complex.
DR   DIP; DIP-9093N; -.
DR   IntAct; P32721; 5.
DR   STRING; 511145.b4087; -.
DR   TCDB; 3.A.1.2.6; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P32721; -.
DR   PRIDE; P32721; -.
DR   EnsemblBacteria; AAC77048; AAC77048; b4087.
DR   EnsemblBacteria; BAE78090; BAE78090; BAE78090.
DR   GeneID; 948593; -.
DR   KEGG; ecj:JW4048; -.
DR   KEGG; eco:b4087; -.
DR   PATRIC; fig|1411691.4.peg.2613; -.
DR   EchoBASE; EB1902; -.
DR   eggNOG; COG1129; Bacteria.
DR   HOGENOM; CLU_000604_92_3_6; -.
DR   InParanoid; P32721; -.
DR   OMA; IDWKEMR; -.
DR   PhylomeDB; P32721; -.
DR   BioCyc; EcoCyc:YJCW-MON; -.
DR   BioCyc; MetaCyc:YJCW-MON; -.
DR   PRO; PR:P32721; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR   GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0015611; F:ABC-type D-ribose transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR   GO; GO:0015754; P:allose transmembrane transport; IC:ComplexPortal.
DR   GO; GO:0015752; P:D-ribose transmembrane transport; IC:ComplexPortal.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Repeat; Sugar transport;
KW   Translocase; Transport.
FT   CHAIN           1..510
FT                   /note="D-allose import ATP-binding protein AlsA"
FT                   /id="PRO_0000091926"
FT   DOMAIN          6..245
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   DOMAIN          260..509
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         38..45
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   510 AA;  56745 MW;  733FA8ABD3618EE1 CRC64;
     MATPYISMAG IGKSFGPVHA LKSVNLTVYP GEIHALLGEN GAGKSTLMKV LSGIHEPTKG
     TITINNISYN KLDHKLAAQL GIGIIYQELS VIDELTVLEN LYIGRHLTKK ICGVNIIDWR
     EMRVRAAMML LRVGLKVDLD EKVANLSISH KQMLEIAKTL MLDAKVIIMD EPTSSLTNKE
     VDYLFLIMNQ LRKEGTAIVY ISHKLAEIRR ICDRYTVMKD GSSVCSGIVS DVSNDDIVRL
     MVGRELQNRF NAMKENVSNL AHETVFEVRN VTSRDRKKVR DISFSVCRGE ILGFAGLVGS
     GRTELMNCLF GVDKRAGGEI RLNGKDISPR SPLDAVKKGM AYITESRRDN GFFPNFSIAQ
     NMAISRSLKD GGYKGAMGLF HEVDEQRTAE NQRELLALKC HSVNQNITEL SGGNQQKVLI
     SKWLCCCPEV IIFDEPTRGI DVGAKAEIYK VMRQLADDGK VILMVSSELP EIITVCDRIA
     VFCEGRLTQI LTNRDDMSEE EIMAWALPQE