ID   3S11_OXYSC              Reviewed;          83 AA.
AC   Q45Z11; Q4VRI1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Short neurotoxin 1;
DE            Short=SNTX-1;
DE   AltName: Full=Toxin 3;
DE   Flags: Precursor;
OS   Oxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Acanthophiinae; Oxyuranus.
OX   NCBI_TaxID=8667;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=16261251; DOI=10.1007/s00018-005-5384-9;
RA   St Pierre L., Woods R., Earl S.T.H., Masci P.P., Lavin M.F.;
RT   "Identification and analysis of venom gland-specific genes from the coastal
RT   taipan (Oxyuranus scutellatus) and related species.";
RL   Cell. Mol. Life Sci. 62:2679-2693(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Welton R.E., Burnell J.N.;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 22-83, FUNCTION, MASS SPECTROMETRY, TOXIC DOSE, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=8672493; DOI=10.1021/bi9600761;
RA   Zamudio F., Wolf K.M., Martin B.M., Possani L.D., Chiappinelli V.A.;
RT   "Two novel alpha-neurotoxins isolated from the taipan snake, Oxyuranus
RT   scutellatus, exhibit reduced affinity for nicotinic acetylcholine receptors
RT   in brain and skeletal muscle.";
RL   Biochemistry 35:7910-7916(1996).
RN   [4]
RP   FUNCTION.
RX   PubMed=17906946; DOI=10.1007/s00018-007-7352-z;
RA   St Pierre L., Fischer H., Adams D.J., Schenning M., Lavidis N.,
RA   de Jersey J., Masci P.P., Lavin M.F.;
RT   "Distinct activities of novel neurotoxins from Australian venomous snakes
RT   for nicotinic acetylcholine receptors.";
RL   Cell. Mol. Life Sci. 64:2829-2840(2007).
CC   -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC       inhibit acetylcholine from binding to the receptor, thereby impairing
CC       neuromuscular transmission. {ECO:0000269|PubMed:17906946,
CC       ECO:0000269|PubMed:8672493}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8672493}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6726; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:8672493};
CC   -!- TOXIC DOSE: LD(50) is 63 ug/kg by intraperitoneal injection into mice.
CC       {ECO:0000269|PubMed:8672493}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; DQ085855; AAZ22673.1; -; mRNA.
DR   EMBL; AY691664; AAY47073.1; -; mRNA.
DR   AlphaFoldDB; Q45Z11; -.
DR   SMR; Q45Z11; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:8672493"
FT   CHAIN           22..83
FT                   /note="Short neurotoxin 1"
FT                   /evidence="ECO:0000269|PubMed:8672493"
FT                   /id="PRO_5000140374"
FT   DISULFID        24..45
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        38..62
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        64..75
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        76..81
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   CONFLICT        57
FT                   /note="I -> R (in Ref. 2; AAY47073)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   83 AA;  9025 MW;  340DADE7A1774781 CRC64;
     MKTLLLTLVV VTIVCLDLGY TMTCYNQQSS EAKTTTTCSG GVSSCYKKTW SDGRGTIIER
     GCGCPSVKKG IERICCRTDK CNN