ID   3S11_PSEAU              Reviewed;          83 AA.
AC   P25497; A8HDJ3;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Short neurotoxin 1;
DE   AltName: Full=Neurotoxin PA A;
DE   Flags: Precursor;
OS   Pseudechis australis (Mulga snake) (King brown snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Acanthophiinae; Pseudechis.
OX   NCBI_TaxID=8670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=17906946; DOI=10.1007/s00018-007-7352-z;
RA   St Pierre L., Fischer H., Adams D.J., Schenning M., Lavidis N.,
RA   de Jersey J., Masci P.P., Lavin M.F.;
RT   "Distinct activities of novel neurotoxins from Australian venomous snakes
RT   for nicotinic acetylcholine receptors.";
RL   Cell. Mol. Life Sci. 64:2829-2840(2007).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-83, FUNCTION, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=4091794; DOI=10.1042/bj2320367;
RA   Takasaki C., Tamiya N.;
RT   "Isolation and amino acid sequence of a short-chain neurotoxin from an
RT   Australian elapid snake, Pseudechis australis.";
RL   Biochem. J. 232:367-371(1985).
CC   -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC       inhibit acetylcholine from binding to the receptor, thereby impairing
CC       neuromuscular transmission. {ECO:0000269|PubMed:4091794}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:4091794}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 0.076 mg/kg by intravenous injection into mice.
CC       {ECO:0000269|PubMed:4091794}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; DQ917498; ABK63527.1; -; mRNA.
DR   AlphaFoldDB; P25497; -.
DR   SMR; P25497; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:4091794"
FT   CHAIN           22..83
FT                   /note="Short neurotoxin 1"
FT                   /evidence="ECO:0000269|PubMed:4091794"
FT                   /id="PRO_5000279906"
FT   DISULFID        24..45
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        38..62
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        64..75
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        76..81
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
SQ   SEQUENCE   83 AA;  9049 MW;  2BE59AF624B7E800 CRC64;
     MKTLLLTLVV VTIVCLDLGY TMTCCNQQSS QPKTTTICAG GESSCYKKTW SDHRGSRTER
     GCGCPHVKPG IKLTCCKTDE CNN