ALT4_ARATH
ID ALT4_ARATH Reviewed; 188 AA.
AC F4HX80; Q9C9G3;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Acyl-acyl carrier protein thioesterase ATL4, chloroplastic {ECO:0000305};
DE EC=3.1.2.- {ECO:0000305};
DE AltName: Full=Acyl-ACP thioesterase ATL4 {ECO:0000305};
DE AltName: Full=Acyl-lipid thioesterase 4 {ECO:0000303|PubMed:24214063};
DE Flags: Precursor;
GN Name=ALT4 {ECO:0000303|PubMed:24214063};
GN OrderedLocusNames=At1g68280 {ECO:0000312|Araport:AT1G68280};
GN ORFNames=T22E19.9 {ECO:0000312|EMBL:AAG52600.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=24214063; DOI=10.1007/s11103-013-0151-z;
RA Pulsifer I.P., Lowe C., Narayaran S.A., Busuttil A.S., Vishwanath S.J.,
RA Domergue F., Rowland O.;
RT "Acyl-lipid thioesterase1-4 from Arabidopsis thaliana form a novel family
RT of fatty acyl-acyl carrier protein thioesterases with divergent expression
RT patterns and substrate specificities.";
RL Plant Mol. Biol. 84:549-563(2014).
CC -!- FUNCTION: Acyl-ACP thioesterase involved in the production of fatty
CC acids and beta-keto fatty acids. Can produce fatty acids of medium to
CC long chain (6:0, 8:0, 10:0 and 16:1) and small amounts of medium to
CC long chain beta-keto fatty acids (8:0, 14:0 and 16:1) when expressed in
CC a heterologous organism (E.coli). Possesses thioesterase activity for
CC lauroyl-ACP (12:0-ACP) in vitro. May play a role in the development of
CC floral organs by generating short chain fatty acids.
CC {ECO:0000269|PubMed:24214063}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:24214063}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in anther walls
CC (endothecium) and in microspores. {ECO:0000269|PubMed:24214063}.
CC -!- SIMILARITY: Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52600.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC016447; AAG52600.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; D96706; D96706.
DR AlphaFoldDB; F4HX80; -.
DR SMR; F4HX80; -.
DR STRING; 3702.AT1G68280.1; -.
DR PaxDb; F4HX80; -.
DR PRIDE; F4HX80; -.
DR Araport; AT1G68280; -.
DR TAIR; locus:2199312; AT1G68280.
DR eggNOG; ENOG502RYRP; Eukaryota.
DR HOGENOM; CLU_101141_1_2_1; -.
DR InParanoid; F4HX80; -.
DR BioCyc; ARA:AT1G68280-MON; -.
DR PRO; PR:F4HX80; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HX80; baseline.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0016297; F:acyl-[acyl-carrier-protein] hydrolase activity; IDA:UniProtKB.
DR GO; GO:0047381; F:dodecanoyl-[acyl-carrier-protein] hydrolase activity; IDA:UniProtKB.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR006683; Thioestr_dom.
DR Pfam; PF03061; 4HBT; 1.
DR SUPFAM; SSF54637; SSF54637; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Hydrolase; Lipid metabolism; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..47
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 48..188
FT /note="Acyl-acyl carrier protein thioesterase ATL4,
FT chloroplastic"
FT /id="PRO_0000435264"
FT ACT_SITE 64
FT /evidence="ECO:0000250|UniProtKB:P56653,
FT ECO:0000250|UniProtKB:Q9C7I5"
SQ SEQUENCE 188 AA; 21318 MW; CD993F75DAB7335F CRC64;
MIRVTGTAAP AMSVVFPTSW RQPVMLPLRS AKTFKPHTFL DLKGGKEMSE FHEVELKVRD
YELDQFGVVN NAVYANYCQH GMHEFLESIG INCDEVARSG EALAISELTM NFLAPLRSGD
KFVVKVNISR TSAARIYFDH SILKLPNQEV ILEAKATVVW LDNKHRPVRI PSSIRSKFVH
FLRQNDTV
