ALTA1_ALTAL
ID ALTA1_ALTAL Reviewed; 157 AA.
AC P79085;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Major allergen Alt a 1;
DE AltName: Allergen=Alt a 1;
DE Flags: Precursor;
GN Name=ALTA1;
OS Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC Alternaria sect. Alternaria; Alternaria alternata complex.
OX NCBI_TaxID=5599;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION,
RP AND ALLERGEN.
RX PubMed=8957113; DOI=10.1159/000237397;
RA De Vouge M.W., Thaker A.J., Curran I.H., Zhang L., Muradia G., Rode H.,
RA Vijay H.M.;
RT "Isolation and expression of a cDNA clone encoding an Alternaria alternata
RT Alt a 1 subunit.";
RL Int. Arch. Allergy Immunol. 111:385-395(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=08-0203-Berlin;
RA Unger A.M., Lechenauer E., Simon B., Oberkofler H., Probst G., Achatz G.,
RA Breitenbach M.;
RL Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBCELLULAR LOCATION, AND ALLERGEN.
RX PubMed=22640235; DOI=10.1111/j.1574-6968.2012.02606.x;
RA Morin M., Asturias J.A., Dominguez A.;
RT "Expression of Alt a 1 allergen from Alternaria alternata in the yeast
RT Yarrowia lipolytica.";
RL FEMS Microbiol. Lett. 333:121-128(2012).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=22078468; DOI=10.1016/j.jaci.2011.10.008;
RA Twaroch T.E., Arcalis E., Sterflinger K., Stoeger E., Swoboda I.,
RA Valenta R.;
RT "Predominant localization of the major Alternaria allergen Alt a 1 in the
RT cell wall of airborne spores.";
RL J. Allergy Clin. Immunol. 129:1148-1149(2012).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24642375; DOI=10.1016/j.febslet.2014.02.044;
RA Gomez-Casado C., Murua-Garcia A., Garrido-Arandia M., Gonzalez-Melendi P.,
RA Sanchez-Monge R., Barber D., Pacios L.F., Diaz-Perales A.;
RT "Alt a 1 from Alternaria interacts with PR5 thaumatin-like proteins.";
RL FEBS Lett. 588:1501-1508(2014).
RN [6]
RP STRUCTURE BY NMR OF 26-157, AND SUBUNIT.
RX PubMed=23715812; DOI=10.1007/s12104-013-9489-z;
RA Wagner G.E., Gutfreund S., Fauland K., Keller W., Valenta R., Zangger K.;
RT "Backbone resonance assignment of Alt a 1, a unique beta-barrel protein and
RT the major allergen of Alternaria alternata.";
RL Biomol. NMR. Assign. 8:229-231(2014).
RN [7] {ECO:0007744|PDB:3V0R}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 26-157, AND SUBUNIT.
RX PubMed=22664167; DOI=10.1016/j.jaci.2012.03.047;
RA Chruszcz M., Chapman M.D., Osinski T., Solberg R., Demas M., Porebski P.J.,
RA Majorek K.A., Pomes A., Minor W.;
RT "Alternaria alternata allergen Alt a 1: a unique beta-barrel protein dimer
RT found exclusively in fungi.";
RL J. Allergy Clin. Immunol. 130:241-247(2012).
RN [8] {ECO:0007744|PDB:4AUD}
RP X-RAY CRYSTALLOGRAPHY (2.67 ANGSTROMS) OF 29-157.
RA Mechaly A.E., Ibanez De Opakua A., Bermejo I., Asturias J., Viguera A.R.;
RT "Crystal Structure of Major Allergen of Alternaria Alternata Alt a 1.";
RL Submitted (MAY-2012) to the PDB data bank.
CC -!- FUNCTION: May bind and inhibit the beta-glucanase activity of host
CC plant thaumatin-like proteins. {ECO:0000269|PubMed:24642375}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000269|PubMed:22664167,
CC ECO:0000269|PubMed:23715812}.
CC -!- INTERACTION:
CC P79085; P81370: tlp; Xeno; NbExp=3; IntAct=EBI-9212161, EBI-9212168;
CC -!- SUBCELLULAR LOCATION: Spore wall {ECO:0000269|PubMed:22078468,
CC ECO:0000269|PubMed:24642375}. Secreted {ECO:0000269|PubMed:22640235,
CC ECO:0000269|PubMed:24642375, ECO:0000269|PubMed:8957113}.
CC -!- ALLERGEN: Causes an allergic reaction in human.
CC {ECO:0000269|PubMed:8957113, ECO:0000305|PubMed:22640235}.
CC -!- SIMILARITY: Belongs to the ALTA1 family. {ECO:0000305}.
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DR EMBL; U86752; AAB47552.1; -; mRNA.
DR EMBL; U82633; AAB40400.1; -; mRNA.
DR RefSeq; XP_018383182.1; XM_018534774.1.
DR PDB; 3V0R; X-ray; 1.90 A; A=26-157.
DR PDB; 4AUD; X-ray; 2.67 A; A/B=29-157.
DR PDBsum; 3V0R; -.
DR PDBsum; 4AUD; -.
DR AlphaFoldDB; P79085; -.
DR BMRB; P79085; -.
DR SMR; P79085; -.
DR IntAct; P79085; 3.
DR MINT; P79085; -.
DR Allergome; 11; Alt a 1.0101.
DR Allergome; 722; Alt a 1.
DR GeneID; 29120368; -.
DR KEGG; aalt:CC77DRAFT_941148; -.
DR OMA; NDQVCET; -.
DR GO; GO:0005619; C:ascospore wall; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR InterPro; IPR032382; AltA1.
DR Pfam; PF16541; AltA1; 1.
DR PROSITE; PS51895; AA1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW Secreted; Signal.
FT SIGNAL 1..18
FT CHAIN 19..157
FT /note="Major allergen Alt a 1"
FT /id="PRO_0000020699"
FT DOMAIN 35..153
FT /note="AA1-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01243"
FT DISULFID 30
FT /note="Interchain"
FT /evidence="ECO:0000269|PubMed:22664167,
FT ECO:0007744|PDB:3V0R"
FT DISULFID 74..89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01243,
FT ECO:0000269|PubMed:22664167, ECO:0007744|PDB:3V0R,
FT ECO:0007744|PDB:4AUD"
FT DISULFID 128..140
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01243,
FT ECO:0000269|PubMed:22664167, ECO:0007744|PDB:3V0R,
FT ECO:0007744|PDB:4AUD"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 39..49
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 56..67
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 71..77
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 87..92
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 96..100
FT /evidence="ECO:0007829|PDB:3V0R"
FT HELIX 101..103
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 105..111
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 117..123
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 126..130
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 132..134
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 138..144
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 146..149
FT /evidence="ECO:0007829|PDB:3V0R"
FT STRAND 151..153
FT /evidence="ECO:0007829|PDB:4AUD"
SQ SEQUENCE 157 AA; 16980 MW; AAC20D0819198246 CRC64;
MQFTTIASLF AAAGLAAAAP LESRQDTASC PVTTEGDYVW KISEFYGRKP EGTYYNSLGF
NIKATNGGTL DFTCSAQADK LEDHKWYSCG ENSFMDFSFD SDRSGLLLKQ KVSDDITYVA
TATLPNYCRA GGNGPKDFVC QGVADAYITL VTLPKSS
