ID   ALX1_XENLA              Reviewed;         335 AA.
AC   Q91574;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=ALX homeobox protein 1 {ECO:0000305};
DE   AltName: Full=Cartilage homeoprotein 1 {ECO:0000250|UniProtKB:Q63087};
DE            Short=CART-1 {ECO:0000250|UniProtKB:Q63087};
DE   AltName: Full=XCART1 {ECO:0000312|EMBL:AAA50368.1};
GN   Name=alx1 {ECO:0000250|UniProtKB:Q15699};
GN   Synonyms=cart1 {ECO:0000250|UniProtKB:Q15699};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Head;
RA   Wright D.A., Allen L.H., de Crombrugghe B.;
RL   Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sequence-specific DNA-binding transcription factor that binds
CC       palindromic sequences within promoters and may activate or repress the
CC       transcription of a subset of genes. Most probably regulates the
CC       expression of genes involved in the development of mesenchyme-derived
CC       craniofacial structures. {ECO:0000250|UniProtKB:Q15699}.
CC   -!- SUBUNIT: Binds DNA as a homodimer; required for transcriptional
CC       activation. {ECO:0000250|UniProtKB:Q63087}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15699}.
CC   -!- DOMAIN: The OAR motif may negatively regulate DNA-binding and therefore
CC       transcriptional activity. It is found in the C-terminal transactivation
CC       domain that stimulates transcription. {ECO:0000250|UniProtKB:Q63087}.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR   EMBL; U15276; AAA50368.1; -; mRNA.
DR   RefSeq; NP_001079116.1; NM_001085647.1.
DR   AlphaFoldDB; Q91574; -.
DR   SMR; Q91574; -.
DR   GeneID; 373650; -.
DR   KEGG; xla:373650; -.
DR   CTD; 373650; -.
DR   Xenbase; XB-GENE-866461; alx1.L.
DR   OrthoDB; 738339at2759; -.
DR   Proteomes; UP000186698; Chromosome 3L.
DR   Bgee; 373650; Expressed in neurula embryo and 5 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0001755; P:neural crest cell migration; IEA:InterPro.
DR   GO; GO:0010718; P:positive regulation of epithelial to mesenchymal transition; ISS:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR033209; ALX1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR003654; OAR_dom.
DR   PANTHER; PTHR24329:SF359; PTHR24329:SF359; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF03826; OAR; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS50803; OAR; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..335
FT                   /note="ALX homeobox protein 1"
FT                   /id="PRO_0000048858"
FT   DNA_BIND        141..200
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          201..335
FT                   /note="Transactivation domain"
FT                   /evidence="ECO:0000250|UniProtKB:Q63087"
FT   MOTIF           315..328
FT                   /note="OAR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
SQ   SEQUENCE   335 AA;  37526 MW;  2DB760F26BC8F3B4 CRC64;
     MDFLTDKFSL KNQPSKAGDF FMGGAGTLEH VMDSMDTESF YSKSPAVVAA AAGASKCVQQ
     GFSAIHRAEH HVRMERASPC QENNANYGLA KVEGQTVHTE LGRSMDTCCS LAVSPGKSMA
     DKVELDELGD KCDSNVSSSK KRRHRTTFTS LQLEELEKVF QKTHYPDVYV REQLALRTEL
     TEARVQVWFQ NRRAKWRKRE RYGQIQQAKS HFAATYDISV LPRADSYPQI QNNLWAGNPS
     GGSVVTSCML PREPSSCMTP YSHSSRTDSP YTGFTNHQNQ FSHMPLNIFF TESLLSGSAN
     GHSFEAKPEF ERRSSSIAVL RMKAKEHTAN ISWAM