ID   ALXM_PHOS4              Reviewed;         285 AA.
AC   P39049;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Alginate lyase;
DE            EC=4.2.2.3;
DE   AltName: Full=Poly(Beta-D-mannuronate) lyase;
DE   AltName: Full=Poly(mana) alginate lyase;
DE   Flags: Precursor;
GN   Name=alxM;
OS   Photobacterium sp. (strain ATCC 43367).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio; Vibrio oreintalis group.
OX   NCBI_TaxID=379097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 21-27.
RX   PubMed=8319887; DOI=10.1111/j.1574-6968.1993.tb06302.x;
RA   Malissard M., Duez C., Guinand M., Vacheron M.-J., Michel G., Marty N.,
RA   Joris B., Thamm I., Ghuysen J.-M.;
RT   "Sequence of a gene encoding a (poly ManA) alginate lyase active on
RT   Pseudomonas aeruginosa alginate.";
RL   FEMS Microbiol. Lett. 110:101-106(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of alginate to give oligosaccharides with
CC         4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing
CC         ends and beta-D-mannuronate at their reducing end.; EC=4.2.2.3;
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 6 family.
CC       {ECO:0000305}.
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DR   EMBL; X70036; CAA49630.1; -; Genomic_DNA.
DR   PIR; S31708; S31708.
DR   AlphaFoldDB; P39049; -.
DR   SMR; P39049; -.
DR   CAZy; PL7; Polysaccharide Lyase Family 7.
DR   BRENDA; 4.2.2.3; 4781.
DR   GO; GO:0045135; F:poly(beta-D-mannuronate) lyase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR014895; Alginate_lyase_2.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   Pfam; PF08787; Alginate_lyase2; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lyase; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:8319887"
FT   CHAIN           21..285
FT                   /note="Alginate lyase"
FT                   /id="PRO_0000024923"
SQ   SEQUENCE   285 AA;  32129 MW;  9F2918C4D8A87812 CRC64;
     MIKSNLVISS LAIVSSMSYA GVEFSNPSGQ LGEPANYTQF ANILSASELQ ISDPNGKKGN
     KEYFALDNDF TGIVNDNFYV DKQSQALVFK MANDHLRNEL RVQKNFRTDL PDHFYTLYAN
     VEILHPLQSM ANSTSKQNEI TFLQVHNKGL DDQGTHNVPH PLLRVVWKEN NQGVKGHFWA
     ITKNNAVICK GSFGKKNKDK EMCRADVAYS KIDLGPAPTD KGTDFTITVG NKTLAIDVNG
     QRKVEKNIDY WRHLLSYFKA GVYNQFTQGE SEAHFNQLRY QVNTP