ID   GLNA1_DICDI             Reviewed;         499 AA.
AC   Q86B00; Q550K6;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Type-1 glutamine synthetase 1;
DE            Short=Type-1 GS 1;
DE            EC=6.3.1.2;
DE   AltName: Full=Type-1 glutamate--ammonia ligase 1;
GN   Name=glnA1; Synonyms=glnA; ORFNames=DDB_G0276835;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   EMBL; AAFI02000019; EAL68919.1; -; Genomic_DNA.
DR   RefSeq; XP_642926.1; XM_637834.1.
DR   AlphaFoldDB; Q86B00; -.
DR   SMR; Q86B00; -.
DR   STRING; 44689.DDB0231552; -.
DR   PaxDb; Q86B00; -.
DR   EnsemblProtists; EAL68919; EAL68919; DDB_G0276835.
DR   GeneID; 8620795; -.
DR   KEGG; ddi:DDB_G0276835; -.
DR   dictyBase; DDB_G0276835; glnA1.
DR   eggNOG; KOG0683; Eukaryota.
DR   HOGENOM; CLU_017290_6_0_1; -.
DR   InParanoid; Q86B00; -.
DR   OMA; TFYTHPE; -.
DR   PhylomeDB; Q86B00; -.
DR   PRO; PR:Q86B00; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..499
FT                   /note="Type-1 glutamine synthetase 1"
FT                   /id="PRO_0000330467"
FT   DOMAIN          50..146
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          158..499
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
SQ   SEQUENCE   499 AA;  56342 MW;  42A903AEE0AA6365 CRC64;
     MIEPQNNKNI NNNIYNNNNN NFKMNQDENQ LILNSKQLLE KIKISKKKNP QLKFIRVCWI
     DISNKIRTKA INIDWILNHE PKLIHVSITN VCMSLLCFED SVTIEALKSE NFGEAFLIPI
     TTTKLNILPY CPSHIQIFGE FFYLDNESKK LKPWLLCPRN SLQRAIDRLK NKFGISLKGS
     FEEEFYLIKK GDNNNSSVAS LLNSIEKLDH GTFANYHSLD CYGDILEKIT NALEEQGLPI
     EQLLSESGSG QFEITIDYTD IMEACDRHII VRQTINSIAS YNGYIATFIP KPFDGLVGSG
     CHAHLSLWDT NDSNLTPDAN GECGLSLVNQ FFIGGLLKHS KSLTALFNTT PNSYKRLKPF
     YWSGCNVSWG LDNKESFIRI PSSPFSATDG CSNFEIKTID HTSNPYLAMA GIIHAGFDGI
     ENSIAPPPPT SLFDQSVLNN QLIPSNFEDA IQSLKENHYL CENIGNDISN AFIHVKLAEN
     KILKELSDDD QILKLLELF