ID   GLNA1_SOYBN             Reviewed;         355 AA.
AC   P24099;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Glutamine synthetase cytosolic isozyme 1;
DE            EC=6.3.1.2;
DE   AltName: Full=GS1-1;
DE   AltName: Full=Glutamate--ammonia ligase;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Prize;
RX   PubMed=1688099; DOI=10.2307/3869196;
RA   Miao G.-H., Hirel B., Marsolier M.C., Ridge R.W., Verma D.P.S.;
RT   "Ammonia-regulated expression of a soybean gene encoding cytosolic
RT   glutamine synthetase in transgenic Lotus corniculatus.";
RL   Plant Cell 3:11-22(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 333-355.
RC   STRAIN=cv. Prize; TISSUE=Root nodule;
RX   PubMed=1356501; DOI=10.1007/bf00014489;
RA   Hirel B., Marsolier M.C., Hoarau A., Hoarau J., Brangeon J., Schafer R.,
RA   Verma D.P.S.;
RT   "Forcing expression of a soybean root glutamine synthetase gene in tobacco
RT   leaves induces a native gene encoding cytosolic enzyme.";
RL   Plant Mol. Biol. 20:207-218(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SUBUNIT: Homooctamer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Irreversibly inhibited by the herbicide L-
CC       phosphinothricin (PPT).
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   EMBL; S46513; AAB23379.1; -; mRNA.
DR   EMBL; X68598; CAA48590.1; -; mRNA.
DR   PIR; JQ0937; JQ0937.
DR   RefSeq; NP_001238531.1; NM_001251602.1.
DR   AlphaFoldDB; P24099; -.
DR   SMR; P24099; -.
DR   STRING; 3847.GLYMA11G33560.1; -.
DR   PRIDE; P24099; -.
DR   GeneID; 548082; -.
DR   KEGG; gmx:548082; -.
DR   eggNOG; KOG0683; Eukaryota.
DR   OrthoDB; 784869at2759; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IBA:GO_Central.
DR   GO; GO:0006542; P:glutamine biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.10.20.70; -; 1.
DR   InterPro; IPR036651; Gln_synt_N.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   InterPro; IPR027303; Gln_synth_gly_rich_site.
DR   InterPro; IPR027302; Gln_synth_N_conserv_site.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF54368; SSF54368; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   PROSITE; PS00180; GLNA_1; 1.
DR   PROSITE; PS00181; GLNA_ATP; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nitrogen fixation; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..355
FT                   /note="Glutamine synthetase cytosolic isozyme 1"
FT                   /id="PRO_0000153196"
FT   DOMAIN          19..98
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          105..355
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
FT   REGION          39..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..66
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   355 AA;  38845 MW;  01A985684B1EF51D CRC64;
     MSLLSDLINL NLSDTTEKVI AEYIWIGGSG MDLRSKARTL PGPVSDPSEL PKWNYDGSST
     GQAPGEDSEA IYTHKPFQDP FRRGNNILVI CDAYTPAGEP IPTNKRHAAA KVFSHPDVVA
     EVPWYGIEQE YTLLQKDIQW PLGWPVGGFP GPQGPYYCGV GADKAFGRDI VDAHYKACIY
     AGINISGING EVMPGQWEFQ VGPSVGISAG DEIWAARYIL ERITEIAGVV VSFDPKPIPG
     DWNGAGAHTN YSTKSMREDG GYEVIKAAID KLGKKHKEHI AAYGEGNERR LTGRHETADI
     NTFLWGVANR GASVRVGRDT EKAGKGYFED RRPASNMDPY VVTSMIADTT ILWKP