ALX_ECOLI
ID ALX_ECOLI Reviewed; 321 AA.
AC P42601; Q2M9B8; Q6BF48;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Putative membrane-bound redox modulator Alx;
GN Name=alx {ECO:0000303|PubMed:2108134}; Synonyms=ygjT;
GN OrderedLocusNames=b3088, JW5515;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP SEQUENCE REVISION TO 172-179.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP GENE NAME, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=2108134; DOI=10.1128/jb.172.4.2184-2186.1990;
RA Bingham R.J., Hall K.S., Slonczewski J.L.;
RT "Alkaline induction of a novel gene locus, alx, in Escherichia coli.";
RL J. Bacteriol. 172:2184-2186(1990).
RN [5]
RP POSSIBLE FUNCTION, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=12107143; DOI=10.1128/jb.184.15.4246-4258.2002;
RA Stancik L.M., Stancik D.M., Schmidt B., Barnhart D.M., Yoncheva Y.N.,
RA Slonczewski J.L.;
RT "pH-dependent expression of periplasmic proteins and amino acid catabolism
RT in Escherichia coli.";
RL J. Bacteriol. 184:4246-4258(2002).
RN [6]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Has been proposed to be a redox modulator.
CC {ECO:0000305|PubMed:12107143}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:15919996}.
CC -!- INDUCTION: By extreme alkaline conditions.
CC {ECO:0000269|PubMed:12107143, ECO:0000269|PubMed:2108134}.
CC -!- SIMILARITY: Belongs to the TerC family. {ECO:0000305}.
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DR EMBL; U18997; AAA57890.1; -; Genomic_DNA.
DR EMBL; U00096; AAT48166.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77138.1; -; Genomic_DNA.
DR PIR; E65097; E65097.
DR RefSeq; WP_001098806.1; NZ_STEB01000001.1.
DR RefSeq; YP_026201.1; NC_000913.3.
DR AlphaFoldDB; P42601; -.
DR BioGRID; 4262405; 14.
DR STRING; 511145.b3088; -.
DR TCDB; 2.A.109.1.7; the tellurium ion resistance (terc) family.
DR PaxDb; P42601; -.
DR PRIDE; P42601; -.
DR EnsemblBacteria; AAT48166; AAT48166; b3088.
DR EnsemblBacteria; BAE77138; BAE77138; BAE77138.
DR GeneID; 947607; -.
DR KEGG; ecj:JW5515; -.
DR KEGG; eco:b3088; -.
DR PATRIC; fig|1411691.4.peg.3641; -.
DR EchoBASE; EB2589; -.
DR eggNOG; COG0861; Bacteria.
DR HOGENOM; CLU_045644_1_2_6; -.
DR InParanoid; P42601; -.
DR OMA; ADHAREY; -.
DR PhylomeDB; P42601; -.
DR BioCyc; EcoCyc:G7607-MON; -.
DR PRO; PR:P42601; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0071467; P:cellular response to pH; IDA:EcoCyc.
DR InterPro; IPR005496; Integral_membrane_TerC.
DR InterPro; IPR022369; Integral_membrane_TerC_rswitch.
DR PANTHER; PTHR30238:SF0; PTHR30238:SF0; 1.
DR Pfam; PF03741; TerC; 1.
DR TIGRFAMs; TIGR03718; R_switched_Alx; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..321
FT /note="Putative membrane-bound redox modulator Alx"
FT /id="PRO_0000103410"
FT TOPO_DOM 1..6
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..89
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..113
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..198
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..225
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..286
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..321
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:15919996"
FT CONFLICT 172..179
FT /note="PLVRWLRG -> RWCAGYAV (in Ref. 1; AAA57890)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 321 AA; 35909 MW; B6E1C173442799C3 CRC64;
MNTVGTPLLW GGFAVVVAIM LAIDLLLQGR RGAHAMTMKQ AAAWSLVWVT LSLLFNAAFW
WYLVQTEGRA VADPQALAFL TGYLIEKSLA VDNVFVWLML FSYFSVPAAL QRRVLVYGVL
GAIVLRTIMI FTGSWLISQF DWILYIFGAF LLFTGVKMAL AHEDESGIGD KPLVRWLRGH
LRMTDTIDNE HFFVRKNGLL YATPLMLVLI LVELSDVIFA VDSIPAIFAV TTDPFIVLTS
NLFAILGLRA MYFLLAGVAE RFSMLKYGLA VILVFIGIKM LIVDFYHIPI AVSLGVVFGI
LVMTFIINAW VNYRHDKQRG G
