ID   GLNA2_PINPS             Reviewed;          15 AA.
AC   P81107;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Probable glutamine synthetase leaf isozyme;
DE            EC=6.3.1.2;
DE   AltName: Full=Glutamate--ammonia ligase;
DE   AltName: Full=N47/N48;
DE   AltName: Full=S2205/S2287;
DE   Flags: Fragment;
OS   Pinus pinaster (Maritime pine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Pinus.
OX   NCBI_TaxID=71647;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Needle;
RA   Plomion C., Costa P., Bahrman N., Frigerio J.-M.;
RT   "Genetic analysis of needle proteins in maritime pine. 1. Mapping dominant
RT   and codominant protein markers assayed on diploid tissue, in a haploid-
RT   based genetic map.";
RL   Silvae Genet. 46:161-165(1997).
RN   [2]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Needle;
RX   PubMed=10344291;
RX   DOI=10.1002/(sici)1522-2683(19990101)20:4/5<1098::aid-elps1098>3.0.co;2-z;
RA   Costa P., Pionneau C., Bauw G., Dubos C., Bahrman N., Kremer A.,
RA   Frigerio J.-M., Plomion C.;
RT   "Separation and characterization of needle and xylem maritime pine
RT   proteins.";
RL   Electrophoresis 20:1098-1108(1999).
CC   -!- FUNCTION: The light-modulated chloroplast enzyme, encoded by a nuclear
CC       gene and expressed primarily in leaves, is responsible for the
CC       reassimilation of the ammonia generated by photorespiration.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 5.7,
CC       its MW is: 42 kDa.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   BRENDA; 6.3.1.2; 4852.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   ATP-binding; Chloroplast; Direct protein sequencing; Ligase;
KW   Nucleotide-binding; Plastid.
FT   CHAIN           <1..>15
FT                   /note="Probable glutamine synthetase leaf isozyme"
FT                   /id="PRO_0000153194"
FT   NON_TER         1
FT   NON_TER         15
SQ   SEQUENCE   15 AA;  1614 MW;  24A2420BEFD60D27 CRC64;
     DVNWPLGWPV GGYPG