GLNA3_DICDI
ID GLNA3_DICDI Reviewed; 735 AA.
AC Q54WR9;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Type-3 glutamine synthetase;
DE EC=6.3.1.2;
DE AltName: Full=Type-3 glutamate--ammonia ligase;
DE Short=Type-3 GS;
GN Name=glnA3; Synonyms=glnB; ORFNames=DDB_G0279591;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glutamine synthetase family. Type 3
CC subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000031; EAL67734.1; -; Genomic_DNA.
DR RefSeq; XP_641648.1; XM_636556.1.
DR AlphaFoldDB; Q54WR9; -.
DR SMR; Q54WR9; -.
DR STRING; 44689.DDB0231551; -.
DR PaxDb; Q54WR9; -.
DR EnsemblProtists; EAL67734; EAL67734; DDB_G0279591.
DR GeneID; 8622055; -.
DR KEGG; ddi:DDB_G0279591; -.
DR dictyBase; DDB_G0279591; glnA3.
DR eggNOG; ENOG502QQE3; Eukaryota.
DR HOGENOM; CLU_024307_0_0_1; -.
DR InParanoid; Q54WR9; -.
DR OMA; QFLVFCA; -.
DR PhylomeDB; Q54WR9; -.
DR PRO; PR:Q54WR9; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR InterPro; IPR040577; Gln-synt_C.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR008146; Gln_synth_cat_dom.
DR InterPro; IPR027303; Gln_synth_gly_rich_site.
DR InterPro; IPR022147; GSIII_N.
DR Pfam; PF00120; Gln-synt_C; 1.
DR Pfam; PF18318; Gln-synt_C-ter; 1.
DR Pfam; PF12437; GSIII_N; 1.
DR SMART; SM01230; Gln-synt_C; 1.
DR SUPFAM; SSF55931; SSF55931; 1.
DR PROSITE; PS00181; GLNA_ATP; 1.
DR PROSITE; PS51986; GS_BETA_GRASP; 1.
DR PROSITE; PS51987; GS_CATALYTIC; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Ligase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..735
FT /note="Type-3 glutamine synthetase"
FT /id="PRO_0000330468"
FT DOMAIN 89..183
FT /note="GS beta-grasp"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT DOMAIN 188..621
FT /note="GS catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
SQ SEQUENCE 735 AA; 82616 MW; 9B2B44F894566A08 CRC64;
MSNRRREFIE YIDSREPVEA DIDISDKADR LTDIYDSDTY GLNAMRETLP SHCYKKIREV
MQTGSALDPE IADMVANGMK EWAIKQGATH YCHWFLPLNG LAAEKHDSFI SIFPGDDKVL
LEFSGMQLIK GEPDASSFPS GGIRSTWEAR GYTVWDATSP AFIRREKNGA ILCIPTAFCS
WTGEALDQKT PLLRSMEYVS NESIITLSSL FNEKHKRISP TLGIEQEFFL IDRKFYLARP
DLVNCGRTLI GAKPPKGQEM EDHYFGTMNS RIISCIQEVE WKMWRLGMPL KTRHNEVAPG
QYEVAPIFER ANIAADHNMM LMDILKNVST KHGLVCLFHE KPFAGVNGSG KHNNWSLSTD
GGSNLLEPGH TPSQNARFIL FLTAIIRAVD IHADLLRASV AVPGNEHRLG ANEAPPAIIS
IYLGKELDTV INNIINNTDI QAPGSDDMDL GVVGFPPLPK DSTDRNRTSP FAFTGNKFEF
RAVGSSQVVN FPCIVLNTIV AESLRFIREE ILREMKVSSR QTAFNKIIKD TLIQHVRVVF
NGDGYSGDWK ELAKSRGLAN LPSTPEALTN INSEKNIKLF SESNILSPVE LESRQEILFE
IYNKSIKIEA NSLYDLVSTL VLPACFAHQK NIAESVNSIM PFIQSQKSFS QPNHQYSHLS
EVVESVNLLI EANQKLLALI KQTKDFNSEH SLATFLNQSV IPQMNEVRKF SDHLEGIVED
KSWPVPKYSE ILFLR