ID   GLNA3_LUPAN             Reviewed;         315 AA.
AC   P14636;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Glutamine synthetase nodule isozyme;
DE            EC=6.3.1.2;
DE   AltName: Full=Glutamate--ammonia ligase;
DE   Flags: Fragment;
OS   Lupinus angustifolius (Narrow-leaved blue lupine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   genistoids sensu lato; core genistoids; Genisteae; Lupinus.
OX   NCBI_TaxID=3871;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Uniharvest; TISSUE=Root nodule;
RX   PubMed=2577495; DOI=10.1007/bf00027308;
RA   Grant M.R., Carne A., Hill D.F., Farnden K.J.F.;
RT   "The isolation and characterization of a cDNA clone encoding Lupinus
RT   angustifolius root nodule glutamine synthetase.";
RL   Plant Mol. Biol. 13:481-490(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SUBUNIT: Homooctamer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   EMBL; X15578; CAA33605.1; -; mRNA.
DR   PIR; S07462; AJYLNB.
DR   AlphaFoldDB; P14636; -.
DR   SMR; P14636; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.10.20.70; -; 1.
DR   InterPro; IPR008147; Gln_synt_b-grasp.
DR   InterPro; IPR036651; Gln_synt_N.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   InterPro; IPR027303; Gln_synth_gly_rich_site.
DR   InterPro; IPR027302; Gln_synth_N_conserv_site.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   Pfam; PF03951; Gln-synt_N; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF54368; SSF54368; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   PROSITE; PS00180; GLNA_1; 1.
DR   PROSITE; PS00181; GLNA_ATP; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nitrogen fixation; Nucleotide-binding.
FT   CHAIN           1..>315
FT                   /note="Glutamine synthetase nodule isozyme"
FT                   /id="PRO_0000153176"
FT   DOMAIN          19..99
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          106..315
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
FT   NON_TER         315
SQ   SEQUENCE   315 AA;  34441 MW;  DC3B61A903F442F3 CRC64;
     MSLLSDLINL NLSDTTEKII AEYIWVGGSG VDLRSKARTL SGPVNDPSKL PKWNYDGSST
     GQAPGKDSEV ILWPQAIFKD PFRRGNNILV ICDTYTPSGK PIPTNKRHAA AKIFSHPDVA
     AEEPWFGIEQ EYTLLQKDIH WPIGMALGGF PGPQGPYYCG TGAEKAFGRD IVDSHYKACL
     YAGINISGIN AEVMPGQWEF QVGPSIGISA GDELWVARYI LERITEIAGV VLSLDPKPIP
     GDWNGAGAHT NYSTKSMRND GGYEVIKQAI EKLEKRHNEH IAAYGEGNER RLTGRHETAD
     ISTFSWGVAN RGASI