GLNAC_MAIZE
ID GLNAC_MAIZE Reviewed; 423 AA.
AC P25462;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Glutamine synthetase, chloroplastic;
DE EC=6.3.1.2;
DE AltName: Full=GS2;
DE AltName: Full=Glutamate--ammonia ligase;
DE Flags: Precursor;
GN Name=GLN2;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Black Mexican Sweet; TISSUE=Leaf;
RX PubMed=2906306; DOI=10.1093/genetics/120.4.1111;
RA Snustad D.P., Hunsperger J.P., Chereskin B.M., Messing J.;
RT "Maize glutamine synthetase cDNAs: isolation by direct genetic selection in
RT Escherichia coli.";
RL Genetics 120:1111-1124(1988).
RN [2]
RP POSSIBLE FRAMESHIFT ERROR IN SEQUENCE DESCRIBED IN PUBMED:2906306.
RA Patthey J.-P.;
RL Unpublished observations (MAY-1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Black Mexican Sweet; TISSUE=Seedling;
RX PubMed=8106013; DOI=10.1007/bf00029015;
RA Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.;
RT "Differential expression of six glutamine synthetase genes in Zea mays.";
RL Plant Mol. Biol. 23:401-407(1993).
CC -!- FUNCTION: The light-modulated chloroplast enzyme, encoded by a nuclear
CC gene and expressed primarily in leaves, is responsible for the
CC reassimilation of the ammonia generated by photorespiration.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC -!- SUBUNIT: Homooctamer.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR EMBL; X65931; CAA46724.1; -; mRNA.
DR PIR; S39482; S39482.
DR RefSeq; NP_001105725.1; NM_001112255.1.
DR AlphaFoldDB; P25462; -.
DR SMR; P25462; -.
DR STRING; 4577.GRMZM2G098290_P02; -.
DR BindingDB; P25462; -.
DR ChEMBL; CHEMBL2285357; -.
DR PaxDb; P25462; -.
DR PRIDE; P25462; -.
DR GeneID; 542746; -.
DR MaizeGDB; 61728; -.
DR eggNOG; KOG0683; Eukaryota.
DR OrthoDB; 784869at2759; -.
DR BRENDA; 6.3.1.2; 6752.
DR PRO; PR:P25462; -.
DR Proteomes; UP000007305; Unplaced.
DR ExpressionAtlas; P25462; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
DR GO; GO:0046686; P:response to cadmium ion; IEA:EnsemblPlants.
DR Gene3D; 3.10.20.70; -; 1.
DR InterPro; IPR008147; Gln_synt_b-grasp.
DR InterPro; IPR036651; Gln_synt_N.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR008146; Gln_synth_cat_dom.
DR InterPro; IPR027303; Gln_synth_gly_rich_site.
DR InterPro; IPR027302; Gln_synth_N_conserv_site.
DR Pfam; PF00120; Gln-synt_C; 1.
DR Pfam; PF03951; Gln-synt_N; 1.
DR SMART; SM01230; Gln-synt_C; 1.
DR SUPFAM; SSF54368; SSF54368; 1.
DR SUPFAM; SSF55931; SSF55931; 1.
DR PROSITE; PS00180; GLNA_1; 1.
DR PROSITE; PS00181; GLNA_ATP; 1.
DR PROSITE; PS51986; GS_BETA_GRASP; 1.
DR PROSITE; PS51987; GS_CATALYTIC; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Ligase; Nucleotide-binding; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 52..423
FT /note="Glutamine synthetase, chloroplastic"
FT /id="PRO_0000011179"
FT DOMAIN 70..150
FT /note="GS beta-grasp"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT DOMAIN 154..423
FT /note="GS catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
FT REGION 89..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 423 AA; 46017 MW; EC799D71E0BD6C5F CRC64;
MAQAVVPAMQ CRVGVKAAAG RVWSAGRTRT GRGGASPGFK VMAVSTGSTG VVPRLEQLLN
MDTTPYTDKV IAEYIWVGGS GIDIRSKSRT ISKPVEDPSE LPKWNYDGSS TGQAPGEDSE
VILYPQAIFK DPFRGGNNVL VICDTYTPQG EPLPTNKRHR AAQIFSDPKV GEQVPWFGIE
QEYTLLQKDV NWPLGWPVGG FPGPQGPYYC AVGADKSFGR DISDAHYKAC LYAGINISGT
NGEVMPGQWE YQVGPSVGIE AGDHIWISRY ILERITEQAG VVLTLDPKPI QGDWNGAGCH
TNYSTKTMRE DGGFEEIKRA ILNLSLRHDL HISAYGEGNE RRLTGKHETA SIGTFSWGVA
NRGCSIRVGR DTEAKGKGYL EDRRPASNMD PYIVTGLLAE TTILWQPSLE AEALAAKKLA
LKV