ID   GLNA_CAEEL              Reviewed;         367 AA.
AC   P34497;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Probable glutamine synthetase;
DE            EC=6.3.1.2;
DE   AltName: Full=Glutamate--ammonia ligase;
GN   Name=gln-2; ORFNames=K03H1.1;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=7906398; DOI=10.1038/368032a0;
RA   Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA   Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA   Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA   Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA   Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA   Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA   Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA   Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA   Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA   Wilkinson-Sproat J., Wohldman P.;
RT   "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT   elegans.";
RL   Nature 368:32-38(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z29560; CAA82655.1; -; Genomic_DNA.
DR   PIR; S41024; S41024.
DR   RefSeq; NP_499208.1; NM_066807.3.
DR   AlphaFoldDB; P34497; -.
DR   SMR; P34497; -.
DR   BioGRID; 41601; 2.
DR   IntAct; P34497; 2.
DR   STRING; 6239.K03H1.1; -.
DR   EPD; P34497; -.
DR   PaxDb; P34497; -.
DR   PeptideAtlas; P34497; -.
DR   EnsemblMetazoa; K03H1.1.1; K03H1.1.1; WBGene00001603.
DR   GeneID; 176407; -.
DR   KEGG; cel:CELE_K03H1.1; -.
DR   UCSC; K03H1.1; c. elegans.
DR   CTD; 176407; -.
DR   WormBase; K03H1.1; CE00507; WBGene00001603; gln-2.
DR   eggNOG; KOG0683; Eukaryota.
DR   HOGENOM; CLU_036762_1_1_1; -.
DR   InParanoid; P34497; -.
DR   OMA; HAVACLY; -.
DR   OrthoDB; 784869at2759; -.
DR   PhylomeDB; P34497; -.
DR   Reactome; R-CEL-210455; Astrocytic Glutamate-Glutamine Uptake And Metabolism.
DR   Reactome; R-CEL-8964539; Glutamate and glutamine metabolism.
DR   PRO; PR:P34497; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00001603; Expressed in material anatomical entity and 5 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IBA:GO_Central.
DR   GO; GO:0006542; P:glutamine biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.10.20.70; -; 1.
DR   InterPro; IPR008147; Gln_synt_b-grasp.
DR   InterPro; IPR036651; Gln_synt_N.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   InterPro; IPR027302; Gln_synth_N_conserv_site.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   Pfam; PF03951; Gln-synt_N; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF54368; SSF54368; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   PROSITE; PS00180; GLNA_1; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..367
FT                   /note="Probable glutamine synthetase"
FT                   /id="PRO_0000153147"
FT   DOMAIN          30..110
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          117..367
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
SQ   SEQUENCE   367 AA;  41284 MW;  A7E0A3351F6D32F6 CRC64;
     MTHLNFETRM PLGTAVIDQF LGLRPHPTKI QATYVWIDGT GENLRSKTRT FDKLPKRIED
     YPIWNYDGSS TGQAKGRDSD RYLRPVAAYP DPFLGGGNKL VMCDTLDHQM QPTATSHRQA
     CAEIMHEIRD TRPWFGMEQE YLIVDRDEHP LGWPKHGFPD PQGKYYCSVG ADRAFGREVV
     ETHYRACLHA GLNIFGTNAE VTPGQWEFQI GTCEGIDMGD QLWMSRYILH RVAEQFGVCV
     SLDPKPKVTM GDWNGAGCHT NFSTAEMRAP GGIAAIEAAM TGLKRTHLEA MKVYDPHGGE
     DNLRRLTGRH ETSSADKFSW GVANRGCSIR IPRQVAAERK GYLEDRRPSS NCDPYQVTAM
     IAQSILF