3S12_AIPLA
ID 3S12_AIPLA Reviewed; 81 AA.
AC P19959; P01439;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Short neurotoxin B;
DE Flags: Precursor;
OS Aipysurus laevis (Olive sea snake).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Hydrophiidae; Aipysurus.
OX NCBI_TaxID=8678;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=2330602; DOI=10.1016/0041-0101(90)90013-w;
RA Ducancel F., Guignery-Frelat G., Boulain J.-C., Menez A.;
RT "Nucleotide sequence and structure analysis of cDNAs encoding short-chain
RT neurotoxins from venom glands of a sea snake (Aipysurus laevis).";
RL Toxicon 28:119-123(1990).
RN [2]
RP PROTEIN SEQUENCE OF 22-81, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=1259717; DOI=10.1042/bj1530079;
RA Maeda N., Tamiya N.;
RT "Isolation, properties and amino acid sequences of three neurotoxins from
RT the venom of a sea snake, Aipysurus laevis.";
RL Biochem. J. 153:79-87(1976).
CC -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC inhibit acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular transmission. {ECO:0000250|UniProtKB:P60775}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1259717}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- TOXIC DOSE: LD(50) is 0.067 mg/kg by intramuscular injection into mice.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR EMBL; X13373; CAA31748.1; -; mRNA.
DR PIR; A34019; A34019.
DR AlphaFoldDB; P19959; -.
DR SMR; P19959; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:1259717"
FT CHAIN 22..81
FT /note="Short neurotoxin B"
FT /evidence="ECO:0000269|PubMed:1259717"
FT /id="PRO_0000035433"
FT DISULFID 24..43
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 38..60
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 62..73
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 74..79
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
SQ SEQUENCE 81 AA; 9048 MW; E9D6403A5D59CE43 CRC64;
MKTLLLTLVV VTIVCLDLGY TLTCCNQQSS QPKTTTDCAD NSCYKMTWRD HRGTRIERGC
GCPQVKPGIK LECCKTNECN N