ID   GLNA_SUIBO              Reviewed;         354 AA.
AC   Q8J1R3;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Glutamine synthetase;
DE            Short=GS;
DE            EC=6.3.1.2;
DE   AltName: Full=Glutamate--ammonia ligase;
GN   Name=GLNA;
OS   Suillus bovinus (Jersey cow bolete) (Boletus bovinus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Boletales; Suillineae; Suillaceae; Suillus.
OX   NCBI_TaxID=48563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SBH1;
RA   Jokela S., Paulin L., Sen R., Juuti J.T.;
RT   "Glutamine synthetase gene and its transcription in the ectomycorrhizal
RT   basidiomycete Suillus bovinus.";
RL   Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   EMBL; AJ509092; CAD48934.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8J1R3; -.
DR   SMR; Q8J1R3; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.10.20.70; -; 1.
DR   InterPro; IPR008147; Gln_synt_b-grasp.
DR   InterPro; IPR036651; Gln_synt_N.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   InterPro; IPR027303; Gln_synth_gly_rich_site.
DR   InterPro; IPR027302; Gln_synth_N_conserv_site.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   Pfam; PF03951; Gln-synt_N; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF54368; SSF54368; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   PROSITE; PS00180; GLNA_1; 1.
DR   PROSITE; PS00181; GLNA_ATP; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN           1..354
FT                   /note="Glutamine synthetase"
FT                   /id="PRO_0000153163"
FT   DOMAIN          22..101
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          108..354
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
SQ   SEQUENCE   354 AA;  39246 MW;  C2AE6EBD4EBDB7D3 CRC64;
     MAYQYHNDLL APYLALSQGD KVQAEYVWID GDGGLRSKTM TMTKKTTDIG QLRIWDFDGS
     STNQAPGGDS DVYLRPAAIF KDPFRGGDNI LVLAETFNND GTPNRTNHRH HTKKVMDLAK
     DSVPWFGLEQ EYTLFDADGT PFGWPKGGFP GPQGPYYCGA GTGKVFARDL IEAHYRACLY
     AGVNISGINA EVMPSQWEFQ VGPCEGISMG DHLWMARYLL VRIAEQWGIK VSFHPKPLAG
     DWNGAGCHTN YSTKVMREPG GMKYIDEAIE KLSKRHAEHI AVYGEDNDLR LTGRHETGHI
     GDFSSGVANR GASIRVPRHV AAQGYGYLED RRPASNIDPY RVTGIIVETT VLDK