GLNB_RHIME
ID GLNB_RHIME Reviewed; 112 AA.
AC Q52905;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Nitrogen regulatory protein P-II;
GN Name=glnB; OrderedLocusNames=R01639; ORFNames=SMc00947;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=8931324; DOI=10.1111/j.1574-6968.1996.tb08553.x;
RA Arcondeguy T., Huez I., Fourment J., Kahn D.;
RT "Symbiotic nitrogen fixation does not require adenylylation of glutamine
RT synthetase I in Rhizobium meliloti.";
RL FEMS Microbiol. Lett. 145:33-40(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: In nitrogen-limiting conditions, when the ratio of Gln to 2-
CC ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP
CC allows the deadenylation of glutamine synthetase (GS), thus activating
CC the enzyme. Conversely, in nitrogen excess P-II is deuridylated and
CC promotes the adenylation of GS. P-II indirectly controls the
CC transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed
CC conversion of NR-I to NR-I-phosphate, the transcriptional activator of
CC glnA. When P-II is uridylylated to P-II-UMP, these events are reversed.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the P(II) protein family. {ECO:0000255|PROSITE-
CC ProRule:PRU00675}.
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DR EMBL; U50385; AAC44623.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC46218.1; -; Genomic_DNA.
DR RefSeq; NP_385745.1; NC_003047.1.
DR RefSeq; WP_003528058.1; NC_003047.1.
DR AlphaFoldDB; Q52905; -.
DR SMR; Q52905; -.
DR STRING; 266834.SMc00947; -.
DR EnsemblBacteria; CAC46218; CAC46218; SMc00947.
DR GeneID; 48973008; -.
DR GeneID; 61603109; -.
DR KEGG; sme:SMc00947; -.
DR PATRIC; fig|266834.11.peg.3072; -.
DR eggNOG; COG0347; Bacteria.
DR HOGENOM; CLU_082268_0_0_5; -.
DR OMA; YRGTEHV; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0030234; F:enzyme regulator activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IEA:InterPro.
DR Gene3D; 3.30.70.120; -; 1.
DR InterPro; IPR002187; N-reg_PII.
DR InterPro; IPR011322; N-reg_PII-like_a/b.
DR InterPro; IPR015867; N-reg_PII/ATP_PRibTrfase_C.
DR InterPro; IPR017918; N-reg_PII_CS.
DR InterPro; IPR002332; N-reg_PII_urydylation_site.
DR PANTHER; PTHR30115; PTHR30115; 1.
DR Pfam; PF00543; P-II; 1.
DR PIRSF; PIRSF039144; GlnB; 1.
DR PRINTS; PR00340; PIIGLNB.
DR SMART; SM00938; P-II; 1.
DR SUPFAM; SSF54913; SSF54913; 1.
DR PROSITE; PS00638; PII_GLNB_CTER; 1.
DR PROSITE; PS51343; PII_GLNB_DOM; 1.
DR PROSITE; PS00496; PII_GLNB_UMP; 1.
PE 3: Inferred from homology;
KW Nitrogen fixation; Nucleotide-binding; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..112
FT /note="Nitrogen regulatory protein P-II"
FT /id="PRO_0000139785"
FT MOD_RES 51
FT /note="O-UMP-tyrosine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00675"
SQ SEQUENCE 112 AA; 12281 MW; 85E6465E64B57001 CRC64;
MKKIEAIIKP FKLDEVKEAL QEVGLQGITV TEAKGFGRQK GHTELYRGAE YVVDFLPKVK
VEVVLADENA EAVIEAIRNA AQTGRIGDGK IFVSNVEEVI RIRTGETGLD AI
