ID   ALYA_KLEPN              Reviewed;         307 AA.
AC   Q59478;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Alginate lyase;
DE            EC=4.2.2.3;
DE   AltName: Full=Poly(beta-D-mannuronate) lyase;
DE   AltName: Full=Poly(mana) alginate lyase;
DE   Flags: Precursor;
GN   Name=alyA;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 21-51.
RC   STRAIN=Subsp. aerogenes 25;
RX   PubMed=8200539; DOI=10.1016/0378-1119(94)90605-x;
RA   Baron A.J., Wong T.Y., Hicks S.J., Gacesa P., Willcock D., McPherson M.J.;
RT   "Alginate lyase from Klebsiella pneumoniae, subsp. aerogenes: gene cloning,
RT   sequence analysis and high-level production in Escherichia coli.";
RL   Gene 143:61-66(1994).
CC   -!- FUNCTION: Degrades alginates that contain guluronic acid.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of alginate to give oligosaccharides with
CC         4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing
CC         ends and beta-D-mannuronate at their reducing end.; EC=4.2.2.3;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 7 family.
CC       {ECO:0000305}.
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DR   EMBL; L19657; AAA25049.1; -; Genomic_DNA.
DR   PDB; 4OZX; X-ray; 1.88 A; A=23-307.
DR   PDBsum; 4OZX; -.
DR   AlphaFoldDB; Q59478; -.
DR   SMR; Q59478; -.
DR   CAZy; PL7; Polysaccharide Lyase Family 7.
DR   BRENDA; 4.2.2.3; 2814.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045135; F:poly(beta-D-mannuronate) lyase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR014895; Alginate_lyase_2.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   Pfam; PF08787; Alginate_lyase2; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Lyase; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:8200539"
FT   CHAIN           21..307
FT                   /note="Alginate lyase"
FT                   /id="PRO_0000024925"
FT   HELIX           25..28
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          34..39
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          44..47
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          49..51
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   HELIX           53..57
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   TURN            63..65
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          66..68
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          74..82
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          93..98
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   TURN            100..103
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          109..113
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          116..118
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   HELIX           124..129
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          130..145
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   TURN            152..156
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          157..164
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          166..168
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          170..176
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          182..190
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          200..206
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          232..239
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          242..248
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          255..260
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          273..281
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          284..286
FT                   /evidence="ECO:0007829|PDB:4OZX"
FT   STRAND          291..302
FT                   /evidence="ECO:0007829|PDB:4OZX"
SQ   SEQUENCE   307 AA;  33512 MW;  76A1F9AAE082632E CRC64;
     MLKSGVMVAS LCLFSVPSRA AVPAPGDKFE LSGWSLSVPV DSDNDGKADQ IKEKTLAAGY
     RNSDFFTLSD AGGMVFKAPI SGAKTSKNTT YTRSELREML RKGDTSIATQ GVSRNNWVLS
     SAPLSEQKKA GGVDGTLEAT LSVDHVTTTG VNWQVGRVII GQIHANNDEP IRLYYRKLPH
     HQKGSVYFAH EPRKGFGDEQ WYEMIGTLQP SHGNQTAAPT EPEAGIALGE TFSYRIDATG
     NKLTVTLMRE GRPDVVKTVD MSKSGYSEAG QYLYFKAGVY NQNKTGKPDD YVQATFYRLK
     ATHGAQR