ID   ALYA_PSEAL              Reviewed;         233 AA.
AC   Q59639;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Alginate lyase;
DE            EC=4.2.2.3;
DE   AltName: Full=Poly(beta-D-mannuronate) lyase;
DE   AltName: Full=Poly(mana) alginate lyase;
DE   Flags: Precursor;
GN   Name=aly;
OS   Pseudomonas alginovora.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=37638;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 24-28; 191-202
RP   AND 208-222.
RC   STRAIN=XO17;
RX   PubMed=8912697; DOI=10.1042/bj3190575;
RA   Chavagnat F., Duez C., Guinand M., Potin P., Barbeyron T., Henrissat B.,
RA   Wallach J., Ghuysen J.-M.;
RT   "Cloning, sequencing and overexpression in Escherichia coli of the
RT   alginatelyase-encoding aly gene of Pseudomonas alginovora: identification
RT   of three classes of alginate lyases.";
RL   Biochem. J. 319:575-583(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of alginate to give oligosaccharides with
CC         4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing
CC         ends and beta-D-mannuronate at their reducing end.; EC=4.2.2.3;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 7 family.
CC       {ECO:0000305}.
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DR   EMBL; X83679; CAA58650.1; -; Genomic_DNA.
DR   PIR; S72449; S72449.
DR   AlphaFoldDB; Q59639; -.
DR   BRENDA; 4.2.2.3; 7127.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045135; F:poly(beta-D-mannuronate) lyase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lyase; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:8912697"
FT   CHAIN           24..233
FT                   /note="Alginate lyase"
FT                   /id="PRO_0000024926"
SQ   SEQUENCE   233 AA;  25873 MW;  6D0CC3AB733E6DDC CRC64;
     MKIISCKSII VSSLLALSAT ATAGSFNDIS WTLENEDNLP ETDASGCALK PSTSTSTSKT
     FEFGLTDDSN CLDGKQRDEF KYQRRTGYNR LTGYFTIDGN YSDFNKMGVA QTHDHSTSDT
     GVFSIYQVRK ENGSYIFGVQ GDSNYSNNGW SDHPQVKISL DTRYELIIKT NGLPNGNSYE
     DANLYLDDVK IWSSSIEVGG EEKQYKKIGA YQLTGGEGEF HVKWDSVKLY TGK