ID   ALYP_PSESO              Reviewed;         398 AA.
AC   Q06365;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Alginate lyase;
DE            EC=4.2.2.3;
DE   AltName: Full=Poly(beta-D-mannuronate) lyase;
DE   AltName: Full=Poly(mana) alginate lyase;
DE   Flags: Precursor;
GN   Name=aly; Synonyms=alyP;
OS   Pseudomonas sp. (strain OS-ALG-9).
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=86038;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8336113; DOI=10.1099/00221287-139-5-987;
RA   Maki H., Mori A., Fujiyama K., Kinoshita S., Yoshida T.;
RT   "Cloning, sequence analysis and expression in Escherichia coli of a gene
RT   encoding an alginate lyase from Pseudomonas sp. OS-ALG-9.";
RL   J. Gen. Microbiol. 139:987-993(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of alginate to give oligosaccharides with
CC         4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing
CC         ends and beta-D-mannuronate at their reducing end.; EC=4.2.2.3;
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 6 family.
CC       {ECO:0000305}.
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DR   EMBL; D10336; BAA01182.1; -; Genomic_DNA.
DR   PIR; S27630; S27630.
DR   AlphaFoldDB; Q06365; -.
DR   SMR; Q06365; -.
DR   CAZy; PL6; Polysaccharide Lyase Family 6.
DR   GO; GO:0045135; F:poly(beta-D-mannuronate) lyase activity; IEA:UniProtKB-EC.
DR   CDD; cd14251; PL-6; 1.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR039513; PL-6.
DR   Pfam; PF14592; Chondroitinas_B; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   3: Inferred from homology;
KW   Lyase; Signal.
FT   SIGNAL          1..?
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..398
FT                   /note="Alginate lyase"
FT                   /id="PRO_0000024924"
SQ   SEQUENCE   398 AA;  44512 MW;  67B4DE1D11998F62 CRC64;
     MPGDKVIMKS GEWKSQFIHF KGKGTAEKPI TLTAETKGSV LLTGNSNLKI DGEWLVVDGL
     SFKNGFSLKD DVVVFTKTTT NSRLTNTSIE NYNPVDKTLD YKWVSLYGHH NRVDHCSITG
     KNHQGTTLVV WLDDKPNYHQ IDHNYFGPRP ELGANGGETI RIGTSAFSMN DSYRTVQNNI
     FDKCDGEVEI ISIKSGFNKI LNNLFYECAG TVTFRHGNNS EVSNNYFIAN NVTNSGGVRI
     IGENQKVYGN YLYKVAGRTL RSAISVMNAY EKPALNDYWQ VKNADIQNNI IVGAREAFVL
     GSGKDNDRTL APDGVNISNN YIINPTTLLV TQDEPKNLKM QNNQVEGASI VTGFVKMGND
     LQMSDGIWQK KTEIKKPFWL ATAIGPEWKK DHRSFIFK