AL_DROME
ID AL_DROME Reviewed; 408 AA.
AC Q06453; Q9VPP1;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Homeobox protein aristaless;
GN Name=al; ORFNames=CG3935;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=8093690; DOI=10.1101/gad.7.1.114;
RA Schneitz K., Spielmann P., Noll M.;
RT "Molecular genetics of aristaless, a prd-type homeo box gene involved in
RT the morphogenesis of proximal and distal pattern elements in a subset of
RT appendages in Drosophila.";
RL Genes Dev. 7:114-129(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Involved in the morphogenesis of proximal and distal pattern
CC elements in a subset of appendages. Also has a role in early imaginal
CC disk development. {ECO:0000269|PubMed:8093690}.
CC -!- INTERACTION:
CC Q06453; P83949: Ubx; NbExp=3; IntAct=EBI-188244, EBI-202590;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00138}.
CC -!- TISSUE SPECIFICITY: Expressed during embryonic development, in distinct
CC epidermal patterns in head, thorax and abdomen and in an endodermal
CC pattern in the anterior intestinal tract. {ECO:0000269|PubMed:8093690}.
CC -!- DEVELOPMENTAL STAGE: First expressed in 4-8 hours old embryos, peaks in
CC 8-12 hours old embryos and continues through development up to late
CC larval stage. In the head region, detected at stage 10 in the maxillary
CC and labial segment primordia, and in later stages, in the prospective
CC antennal and mandibular segment. In the epidermis, expression is seen
CC from stage 11 in thoracic and abdominal lateral patches. Expression in
CC the intestinal tract begins at stage 13, continues through stages 14
CC and 15 in the endoderm of the anterior midgut and at stage 16, is found
CC in the posterior end. Expression in the imaginal disks is seen from
CC late third-instar larvae in the prospective thorax, claw organ,
CC antenna, scutellum and wing blade. {ECO:0000269|PubMed:8093690}.
CC -!- DISRUPTION PHENOTYPE: Flies display a reduction in size of the arista
CC and scutellum, a reduction or complete absence of the tarsal claws,
CC irregularities of the sternopleural bristles and of the wing vein, and
CC a bending of the wing blade. {ECO:0000269|PubMed:8093690}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; L08401; AAA28840.1; -; mRNA.
DR EMBL; AE014134; AAF51505.1; -; Genomic_DNA.
DR EMBL; AY121696; AAM52023.1; -; mRNA.
DR PIR; A40685; A40685.
DR RefSeq; NP_722629.1; NM_164382.2.
DR PDB; 3A01; X-ray; 2.70 A; B/F=80-146.
DR PDB; 3A02; X-ray; 1.00 A; A=91-146.
DR PDB; 3LNQ; X-ray; 2.25 A; A=87-144.
DR PDBsum; 3A01; -.
DR PDBsum; 3A02; -.
DR PDBsum; 3LNQ; -.
DR AlphaFoldDB; Q06453; -.
DR SMR; Q06453; -.
DR BioGRID; 59464; 24.
DR DIP; DIP-23247N; -.
DR IntAct; Q06453; 3.
DR MINT; Q06453; -.
DR STRING; 7227.FBpp0077713; -.
DR PaxDb; Q06453; -.
DR EnsemblMetazoa; FBtr0078053; FBpp0077713; FBgn0000061.
DR GeneID; 33208; -.
DR KEGG; dme:Dmel_CG3935; -.
DR UCSC; CG3935-RA; d. melanogaster.
DR CTD; 33208; -.
DR FlyBase; FBgn0000061; al.
DR VEuPathDB; VectorBase:FBgn0000061; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000165209; -.
DR HOGENOM; CLU_816884_0_0_1; -.
DR InParanoid; Q06453; -.
DR OMA; MASMYSP; -.
DR OrthoDB; 1085093at2759; -.
DR PhylomeDB; Q06453; -.
DR BioGRID-ORCS; 33208; 0 hits in 3 CRISPR screens.
DR EvolutionaryTrace; Q06453; -.
DR GenomeRNAi; 33208; -.
DR PRO; PR:Q06453; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0000061; Expressed in embryonic head and 40 other tissues.
DR Genevisible; Q06453; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048800; P:antennal morphogenesis; IMP:FlyBase.
DR GO; GO:0022416; P:chaeta development; IMP:FlyBase.
DR GO; GO:0035015; P:elongation of arista core; IMP:FlyBase.
DR GO; GO:0007480; P:imaginal disc-derived leg morphogenesis; IMP:FlyBase.
DR GO; GO:0035218; P:leg disc development; IMP:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome.
FT CHAIN 1..408
FT /note="Homeobox protein aristaless"
FT /id="PRO_0000048811"
FT DNA_BIND 85..144
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 50..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 258..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 378..391
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT COMPBIAS 50..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..294
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..309
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 331..354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..371
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 254
FT /note="T -> P (in Ref. 1; AAA28840)"
FT /evidence="ECO:0000305"
FT CONFLICT 321
FT /note="H -> Q (in Ref. 1; AAA28840)"
FT /evidence="ECO:0000305"
FT HELIX 94..106
FT /evidence="ECO:0007829|PDB:3A02"
FT HELIX 112..122
FT /evidence="ECO:0007829|PDB:3A02"
FT HELIX 126..140
FT /evidence="ECO:0007829|PDB:3A02"
SQ SEQUENCE 408 AA; 43657 MW; 7641316A4A77BD8D CRC64;
MGISEEIKLE ELPQEAKLAH PDAVVLVDRA PGSSAASAGA ALTVSMSVSG GAPSGASGAS
GGTNSPVSDG NSDCEADEYA PKRKQRRYRT TFTSFQLEEL EKAFSRTHYP DVFTREELAM
KIGLTEARIQ VWFQNRRAKW RKQEKVGPQS HPYNPYLPGG AATMQTVVGA ALPPNPFTHL
GFQLRKPFDA QHAANLAAFR YPHLSAAPMI PSGYFNQFQR APPHMLPHGM AGMYSPSSSF
QSLLANMTAV PRGTPLGKPP ALLVGSPDLH SPNHMLASPP TSPASGHASQ HQQHPTAHPP
PPQAPPQMPV GVQPAQLSPQ HLVGIALTQQ ASSLSPTQTS PVALTLSHSP QRQLPPPSHQ
APPPPPRAAT PPEDRRTSSI AALRLKAREH ELKLELLRQN GHGNDVVS
