AMA1_YEAST
ID AMA1_YEAST Reviewed; 593 AA.
AC P50082; D6VV07; Q96VQ3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Meiosis-specific APC/C activator protein AMA1;
DE AltName: Full=Activator of meiotic APC/C protein 1;
DE AltName: Full=Sporulation-specific protein 70;
GN Name=AMA1; Synonyms=SPO70; OrderedLocusNames=YGR225W; ORFNames=G8541;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INTERACTION WITH CDC16.
RX PubMed=11114178; DOI=10.1073/pnas.250351297;
RA Cooper K.F., Mallory M.J., Egeland D.B., Jarnik M., Strich R.;
RT "Ama1p is a meiosis-specific regulator of the anaphase promoting
RT complex/cyclosome in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:14548-14553(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8701610;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<385::aid-yea910>3.0.co;2-g;
RA van der Aart Q.J.M., Kleine K., Steensma H.Y.;
RT "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1
RT region from the right arm of Saccharomyces cerevisiae chromosome VII.";
RL Yeast 12:385-390(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=9784122; DOI=10.1126/science.282.5389.699;
RA Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P.O.,
RA Herskowitz I.;
RT "The transcriptional program of sporulation in budding yeast.";
RL Science 282:699-705(1998).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=10734188; DOI=10.1093/nar/28.8.1700;
RA Davis C.A., Grate L., Spingola M., Ares M. Jr.;
RT "Test of intron predictions reveals novel splice sites, alternatively
RT spliced mRNAs and new introns in meiotically regulated genes of yeast.";
RL Nucleic Acids Res. 28:1700-1706(2000).
CC -!- FUNCTION: Activator protein that regulates the ubiquitin ligase
CC activity and substrate specificity of the anaphase promoting
CC complex/cyclosome (APC/C). Required for the ubiquitination and
CC subsequent degradation of the B-type cyclin CLB1 by the APC/C complex
CC during meiosis. Required for meiosis I, late meiotic gene expression
CC and spore wall assembly. {ECO:0000269|PubMed:11114178,
CC ECO:0000269|PubMed:9784122}.
CC -!- SUBUNIT: Interacts with CDC16. {ECO:0000269|PubMed:11114178}.
CC -!- DEVELOPMENTAL STAGE: Expressed and spliced only during meiosis
CC dependent on the splicing factor MER1. {ECO:0000269|PubMed:10734188,
CC ECO:0000269|PubMed:9784122}.
CC -!- DOMAIN: The C-box is required for the association with the APC/C
CC complex. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA61174.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA61174.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA97253.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY033394; AAK61800.1; -; Genomic_DNA.
DR EMBL; X87941; CAA61174.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z73010; CAA97253.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006941; DAA08318.1; -; Genomic_DNA.
DR PIR; S57689; S57689.
DR RefSeq; NP_011741.5; NM_001181354.3.
DR AlphaFoldDB; P50082; -.
DR SMR; P50082; -.
DR BioGRID; 33478; 80.
DR ComplexPortal; CPX-762; Anaphase-Promoting complex AMA1 variant.
DR DIP; DIP-7218N; -.
DR ELM; P50082; -.
DR IntAct; P50082; 3.
DR MINT; P50082; -.
DR STRING; 4932.YGR225W; -.
DR PaxDb; P50082; -.
DR PRIDE; P50082; -.
DR EnsemblFungi; YGR225W_mRNA; YGR225W; YGR225W.
DR GeneID; 853140; -.
DR KEGG; sce:YGR225W; -.
DR SGD; S000003457; AMA1.
DR VEuPathDB; FungiDB:YGR225W; -.
DR eggNOG; KOG0305; Eukaryota.
DR GeneTree; ENSGT00950000183104; -.
DR HOGENOM; CLU_014831_3_3_1; -.
DR InParanoid; P50082; -.
DR OMA; SICIATN; -.
DR BioCyc; YEAST:G3O-30906-MON; -.
DR PRO; PR:P50082; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P50082; protein.
DR GO; GO:0005680; C:anaphase-promoting complex; IPI:SGD.
DR GO; GO:0010997; F:anaphase-promoting complex binding; IBA:GO_Central.
DR GO; GO:1990757; F:ubiquitin ligase activator activity; IDA:SGD.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0030437; P:ascospore formation; IMP:SGD.
DR GO; GO:0030476; P:ascospore wall assembly; IMP:SGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007127; P:meiosis I; IMP:SGD.
DR GO; GO:0045132; P:meiotic chromosome segregation; IMP:SGD.
DR GO; GO:0044778; P:meiotic DNA integrity checkpoint signaling; IMP:SGD.
DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IMP:SGD.
DR GO; GO:1903024; P:positive regulation of ascospore-type prospore membrane formation; IGI:SGD.
DR GO; GO:0045732; P:positive regulation of protein catabolic process; IMP:SGD.
DR GO; GO:1904668; P:positive regulation of ubiquitin protein ligase activity; IDA:SGD.
DR GO; GO:0016567; P:protein ubiquitination; IC:ComplexPortal.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007130; P:synaptonemal complex assembly; IMP:SGD.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR033010; Cdc20/Fizzy.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19918; PTHR19918; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Meiosis; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..593
FT /note="Meiosis-specific APC/C activator protein AMA1"
FT /id="PRO_0000050842"
FT REPEAT 226..264
FT /note="WD 1"
FT REPEAT 271..310
FT /note="WD 2"
FT REPEAT 323..364
FT /note="WD 3"
FT REPEAT 388..427
FT /note="WD 4"
FT REPEAT 432..474
FT /note="WD 5"
FT REPEAT 525..564
FT /note="WD 6"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 29..35
FT /note="C-box"
FT /evidence="ECO:0000250"
FT COMPBIAS 8..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 593 AA; 67027 MW; 35685463E1A677CA CRC64;
MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF DISYSELCEK
SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS ESQVTRSGSA RASRNDYSKL
TKEQKDHRKN IAHSLGFQLP DRVFTFETTS AEILEKNKAI KNCFGPGSCA EIRSTFDFST
LSPDVARYYI ANSNARSASP QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT
NNVLVGLGCS VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY
DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN VSLFEIKSLH
FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE HANLLAVGGN DNSCSLWDIS
DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ
VTSLIWSLRH KQIVATFGFG DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM
AICVATNDET IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR
