ID   3S12_HYDHA              Reviewed;          81 AA.
AC   Q8UW26;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Short neurotoxin 2;
DE   AltName: Full=SN36;
DE   Flags: Precursor;
OS   Hydrophis hardwickii (Hardwick's spine-bellied seasnake) (Lapemis
OS   hardwickii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Hydrophiidae; Hydrophis.
OX   NCBI_TaxID=8781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TOXIC DOSE.
RC   TISSUE=Venom gland;
RX   PubMed=12040423;
RA   Zhong X.F., Peng L.S., Wu W.Y., Wei J.W., Yang H., Yang Y.Z., Xu A.L.;
RT   "Identification and functional characterization of three postsynaptic
RT   short-chain neurotoxins from hydrophiinae, Lapemis hardwickii Gray.";
RL   Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 33:457-462(2001).
CC   -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC       inhibit acetylcholine from binding to the receptor, thereby impairing
CC       neuromuscular transmission. {ECO:0000250|UniProtKB:P60775}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 0.3467 mg/kg by intraperitoneal injection into
CC       mice. {ECO:0000269|PubMed:12040423}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; AF159540; AAL54895.1; -; mRNA.
DR   AlphaFoldDB; Q8UW26; -.
DR   SMR; Q8UW26; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   3: Inferred from homology;
KW   Acetylcholine receptor inhibiting toxin; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Postsynaptic neurotoxin; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..81
FT                   /note="Short neurotoxin 2"
FT                   /id="PRO_0000316172"
FT   DISULFID        24..43
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        38..60
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        62..73
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        74..79
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
SQ   SEQUENCE   81 AA;  9028 MW;  F3F62372BB2ED168 CRC64;
     MKTLLLTLVV VTIVCLDLGY TMTCCNQQSS QPKTTTNCAE SSCYKKTWSD HRGTRIERGC
     GCPQVKHGIK LECCHTNECN N