GLNK2_METJA
ID GLNK2_METJA Reviewed; 112 AA.
AC Q58740;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Nitrogen regulatory protein GlnK2 {ECO:0000250|UniProtKB:Q60381};
GN Name=glnK2 {ECO:0000305}; OrderedLocusNames=MJ1344;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Involved in the regulation of nitrogen metabolism (By
CC similarity). Regulates the activity of its targets by protein-protein
CC interaction in response to the nitrogen status of the cell (By
CC similarity). Regulates the activity of the ammonia channel Amt2 via
CC direct interaction (By similarity). {ECO:0000250|UniProtKB:Q60381}.
CC -!- SUBUNIT: Homotrimer (By similarity). Interacts and forms a complex with
CC Amt2 (By similarity). {ECO:0000250|UniProtKB:Q60381}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B8ZYW0}.
CC -!- SIMILARITY: Belongs to the P(II) protein family. {ECO:0000255|PROSITE-
CC ProRule:PRU00675}.
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DR EMBL; L77117; AAB99353.1; -; Genomic_DNA.
DR PIR; G64467; G64467.
DR RefSeq; WP_010870862.1; NC_000909.1.
DR AlphaFoldDB; Q58740; -.
DR SMR; Q58740; -.
DR STRING; 243232.MJ_1344; -.
DR EnsemblBacteria; AAB99353; AAB99353; MJ_1344.
DR GeneID; 1452247; -.
DR KEGG; mja:MJ_1344; -.
DR eggNOG; arCOG02305; Archaea.
DR HOGENOM; CLU_082268_0_0_2; -.
DR InParanoid; Q58740; -.
DR OMA; YRGTEHV; -.
DR OrthoDB; 113533at2157; -.
DR PhylomeDB; Q58740; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IBA:GO_Central.
DR Gene3D; 3.30.70.120; -; 1.
DR InterPro; IPR002187; N-reg_PII.
DR InterPro; IPR011322; N-reg_PII-like_a/b.
DR InterPro; IPR015867; N-reg_PII/ATP_PRibTrfase_C.
DR InterPro; IPR017918; N-reg_PII_CS.
DR PANTHER; PTHR30115; PTHR30115; 1.
DR Pfam; PF00543; P-II; 1.
DR PRINTS; PR00340; PIIGLNB.
DR SMART; SM00938; P-II; 1.
DR SUPFAM; SSF54913; SSF54913; 1.
DR PROSITE; PS00638; PII_GLNB_CTER; 1.
DR PROSITE; PS51343; PII_GLNB_DOM; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome.
FT CHAIN 1..112
FT /note="Nitrogen regulatory protein GlnK2"
FT /id="PRO_0000139816"
FT BINDING 29
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 37..39
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 37..39
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 64
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 64
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 87..90
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:O28524"
FT BINDING 87..90
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O28524"
SQ SEQUENCE 112 AA; 12532 MW; 3DDCE2A0F32FE9F3 CRC64;
MKKVEAIIRP EKLEIVKKAL SDAGYVGMTV SEVKGRGVQG GIVERYRGRE YIVDLIPKVK
IELVVKEEDV DNVIDIICEN ARTGNPGDGK IFVIPVERVV RVRTKEEGRD VL