AMAL_DROME
ID AMAL_DROME Reviewed; 333 AA.
AC P15364; Q9V3A5;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 18-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Protein amalgam;
DE Flags: Precursor;
GN Name=Ama; ORFNames=CG2198;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=3141062; DOI=10.1016/0092-8674(88)90217-6;
RA Seeger M.A., Haffley L., Kaufman T.C.;
RT "Characterization of amalgam: a member of the immunoglobulin superfamily
RT from Drosophila.";
RL Cell 55:589-600(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Berkeley;
RA Celniker S.E., Pfeiffer B.D., Knafels J., Martin C.H., Mayeda C.A.,
RA Palazzolo M.J.;
RT "Complete sequence of the Antennapedia complex of Drosophila.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
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DR EMBL; M23561; AAA28367.1; -; Genomic_DNA.
DR EMBL; AE001572; AAD19797.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54084.1; -; Genomic_DNA.
DR EMBL; AY051911; AAK93335.1; -; mRNA.
DR PIR; A31923; A31923.
DR RefSeq; NP_476579.1; NM_057231.4.
DR RefSeq; NP_788589.1; NM_176412.2.
DR AlphaFoldDB; P15364; -.
DR SMR; P15364; -.
DR BioGRID; 66029; 18.
DR DIP; DIP-19577N; -.
DR IntAct; P15364; 1.
DR STRING; 7227.FBpp0081135; -.
DR GlyGen; P15364; 3 sites.
DR PaxDb; P15364; -.
DR DNASU; 40831; -.
DR EnsemblMetazoa; FBtr0081619; FBpp0081136; FBgn0000071.
DR EnsemblMetazoa; FBtr0081620; FBpp0081137; FBgn0000071.
DR GeneID; 40831; -.
DR KEGG; dme:Dmel_CG2198; -.
DR UCSC; CG2198-RA; d. melanogaster.
DR CTD; 40831; -.
DR FlyBase; FBgn0000071; Ama.
DR VEuPathDB; VectorBase:FBgn0000071; -.
DR eggNOG; KOG3510; Eukaryota.
DR GeneTree; ENSGT00940000174385; -.
DR HOGENOM; CLU_027228_4_0_1; -.
DR InParanoid; P15364; -.
DR OrthoDB; 583722at2759; -.
DR PhylomeDB; P15364; -.
DR SignaLink; P15364; -.
DR BioGRID-ORCS; 40831; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Ama; fly.
DR GenomeRNAi; 40831; -.
DR PRO; PR:P15364; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000071; Expressed in embryonic/larval hemocyte (Drosophila) and 59 other tissues.
DR ExpressionAtlas; P15364; baseline and differential.
DR Genevisible; P15364; DM.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:0048018; F:receptor ligand activity; IDA:FlyBase.
DR GO; GO:0007157; P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; IPI:FlyBase.
DR GO; GO:0035633; P:maintenance of blood-brain barrier; IMP:FlyBase.
DR GO; GO:0045742; P:positive regulation of epidermal growth factor receptor signaling pathway; IGI:FlyBase.
DR GO; GO:1903977; P:positive regulation of glial cell migration; IMP:FlyBase.
DR GO; GO:0060252; P:positive regulation of glial cell proliferation; IMP:FlyBase.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR Pfam; PF07679; I-set; 2.
DR SMART; SM00409; IG; 3.
DR SMART; SM00408; IGc2; 3.
DR SUPFAM; SSF48726; SSF48726; 3.
DR PROSITE; PS50835; IG_LIKE; 3.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor;
KW Immunoglobulin domain; Lipoprotein; Membrane; Reference proteome; Repeat;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..?
FT /note="Protein amalgam"
FT /id="PRO_0000014504"
FT PROPEP ?..333
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014505"
FT DOMAIN 25..128
FT /note="Ig-like V-type"
FT DOMAIN 139..223
FT /note="Ig-like C2-type 1"
FT DOMAIN 230..323
FT /note="Ig-like C2-type 2"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 308
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..117
FT /evidence="ECO:0000305"
FT DISULFID 161..208
FT /evidence="ECO:0000305"
FT DISULFID 251..307
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="Q -> K (in Ref. 1; AAA28367)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 333 AA; 36387 MW; F644753DE3DB25F1 CRC64;
MARLRLLIGL IFCLAISLDS VLSAPVISQI SKDVVASVGD SVEFNCTVEE VGQLSVSWAK
RPSESDTNSV VLSMRNILSL PDQRYNVTVT EGPKTGSAIY TFRIQNIEVS DMGPYECQVL
VSATEKVTKK LSLQIKTPPV IAENTPKSTL VTEGQNLELT CHANGFPKPT ISWAREHNAV
MPAGGHLLAE PTLRIRSVHR MDRGGYYCIA QNGEGQPDKR LIRVEVEFRP QIAVQRPKIA
QMVSHSAELE CSVQGYPAPT VVWHKNGVPL QSSRHHEVAN TASSSGTTTS VLRIDSVGEE
DFGDYYCNAT NKLGHADARL HLFQTVIPVP SLS
