ID   GLNR_BACCR              Reviewed;         129 AA.
AC   P62174; P19083;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2004, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=HTH-type transcriptional regulator GlnR {ECO:0000250|UniProtKB:P37582};
GN   Name=glnR {ECO:0000250|UniProtKB:P37582}; OrderedLocusNames=BC_3706;
OS   Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS   15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=226900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=12721630; DOI=10.1038/nature01582;
RA   Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA   Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA   Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA   Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT   "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT   anthracis.";
RL   Nature 423:87-91(2003).
CC   -!- FUNCTION: Transcription repressor during nitrogen excess. On the
CC       contrary of the MerR members, which require longer DNA sites for high-
CC       affinity binding, GlnR requires a DNA sequence of 17 nucleotides as
CC       minimal binding site. {ECO:0000250|UniProtKB:P37582}.
CC   -!- ACTIVITY REGULATION: Under conditions of nitrogen excess, the DNA
CC       binding activity of GlnR is activated by a transient interaction with
CC       feedback-inhibited GlnA. Under conditions of nitrogen-limited, GlnR is
CC       autoinhibited by its C-terminal region. {ECO:0000250|UniProtKB:P37582}.
CC   -!- SUBUNIT: Homodimer under conditions of nitrogen excess. Monomer under
CC       conditions of nitrogen-limited. Interacts with feedback-inhibited GlnA
CC       in order to stabilizes GlnR-DNA complex.
CC       {ECO:0000250|UniProtKB:P37582}.
CC   -!- INDUCTION: Under conditions of nitrogen-limited growth, repressed by
CC       TnrA. {ECO:0000250|UniProtKB:P37582}.
CC   -!- MISCELLANEOUS: The amino acid sequences of the N-terminal DNA binding
CC       domains of TnrA and GlnR are highly similar, and both proteins bind to
CC       DNA sequences with a common consensus sequence. In contrast, the C-
CC       terminal signal transduction domains of TnrA and GlnR have no homology.
CC       {ECO:0000250|UniProtKB:P37582}.
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DR   EMBL; AE016877; AAP10634.1; -; Genomic_DNA.
DR   RefSeq; NP_833433.1; NC_004722.1.
DR   RefSeq; WP_000656783.1; NZ_CP034551.1.
DR   AlphaFoldDB; P62174; -.
DR   SMR; P62174; -.
DR   STRING; 226900.BC_3706; -.
DR   EnsemblBacteria; AAP10634; AAP10634; BC_3706.
DR   GeneID; 59155801; -.
DR   GeneID; 64202245; -.
DR   GeneID; 67508342; -.
DR   KEGG; bce:BC3706; -.
DR   PATRIC; fig|226900.8.peg.3815; -.
DR   HOGENOM; CLU_060077_9_0_9; -.
DR   OMA; SIVMQLT; -.
DR   PRO; PR:P62174; -.
DR   Proteomes; UP000001417; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR009061; DNA-bd_dom_put_sf.
DR   InterPro; IPR000551; MerR-type_HTH_dom.
DR   Pfam; PF13411; MerR_1; 1.
DR   SMART; SM00422; HTH_MERR; 1.
DR   SUPFAM; SSF46955; SSF46955; 1.
DR   PROSITE; PS00552; HTH_MERR_1; 1.
DR   PROSITE; PS50937; HTH_MERR_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..129
FT                   /note="HTH-type transcriptional regulator GlnR"
FT                   /id="PRO_0000098121"
FT   DOMAIN          10..78
FT                   /note="HTH merR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   DNA_BIND        13..32
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
SQ   SEQUENCE   129 AA;  15173 MW;  BA363083892C6802 CRC64;
     MKEDRRSAPL FPIGIVMDLT QLSARQIRYY EEHNLVSPTR TKGNRRLFSF NDVDKLLEIK
     DLLDQGLNMA GIKQVLLMKE NQTEAVKVKE ETKEISKTEL RKILRDELQH TGRFNRTSLR
     QGDISRFFH