ID   GLNT_BACSU              Reviewed;         478 AA.
AC   O31464; Q7DL42;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Probable sodium/glutamine symporter GlnT;
GN   Name=glnT; Synonyms=ybgH; OrderedLocusNames=BSU02420;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT   "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT   Bacillus subtilis chromosome.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO N-TERMINUS.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   FUNCTION, AND GENE NAME.
RC   STRAIN=168;
RX   PubMed=15995196; DOI=10.1128/jb.187.14.4813-4821.2005;
RA   Satomura T., Shimura D., Asai K., Sadaie Y., Hirooka K., Fujita Y.;
RT   "Enhancement of glutamine utilization in Bacillus subtilis through the
RT   GlnK-GlnL two-component regulatory system.";
RL   J. Bacteriol. 187:4813-4821(2005).
CC   -!- FUNCTION: Probably functions as a sodium/glutamine symporter for
CC       glutamine uptake. {ECO:0000269|PubMed:15995196}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alanine or glycine:cation symporter (AGCS)
CC       (TC 2.A.25) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA33140.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AB006424; BAA33140.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL009126; CAB12036.2; -; Genomic_DNA.
DR   PIR; C69751; C69751.
DR   RefSeq; NP_388124.2; NC_000964.3.
DR   RefSeq; WP_003246395.1; NZ_JNCM01000030.1.
DR   AlphaFoldDB; O31464; -.
DR   SMR; O31464; -.
DR   STRING; 224308.BSU02420; -.
DR   PaxDb; O31464; -.
DR   PRIDE; O31464; -.
DR   EnsemblBacteria; CAB12036; CAB12036; BSU_02420.
DR   GeneID; 938419; -.
DR   KEGG; bsu:BSU02420; -.
DR   PATRIC; fig|224308.179.peg.249; -.
DR   eggNOG; COG1115; Bacteria.
DR   InParanoid; O31464; -.
DR   OMA; GMATKFV; -.
DR   PhylomeDB; O31464; -.
DR   BioCyc; BSUB:BSU02420-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0015655; F:alanine:sodium symporter activity; IEA:InterPro.
DR   InterPro; IPR001463; Na/Ala_symport.
DR   PANTHER; PTHR30330; PTHR30330; 1.
DR   Pfam; PF01235; Na_Ala_symp; 1.
DR   PRINTS; PR00175; NAALASMPORT.
DR   TIGRFAMs; TIGR00835; agcS; 1.
DR   PROSITE; PS00873; NA_ALANINE_SYMP; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..478
FT                   /note="Probable sodium/glutamine symporter GlnT"
FT                   /id="PRO_0000161565"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        411..431
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   478 AA;  51584 MW;  334C8EDD16C244D6 CRC64;
     MQQILEHIVG IANDLLWSKL LIVLLLSFGI YFTFRLKFLQ VRMLKEMVRV LREGAASRSK
     NSISPFQAFC ISMAARVGTG NITGIAIAIA LGGPGAIFWM WIIAIIGSAS SFVESTLAQI
     YKVKDVNGFR GGPAYYMEKG LNKRWMGALF AVLITLSFGI VFNSVQSNTV SLAFENAFGT
     NRLTLGLILI AVFGTIIFGG VKRIAKLAES IVVVLAVLYI GVAFFVIFSN ITQLPGVLAL
     IVKNAFGFDQ AAGGALGAAL MQGVRRGIFS NEAGMGSAPN AAATATTSHP VKQGLIQAFG
     VLTDTLVICT STAFIILFSD AYHTPGLSGI ALTQASLSSH VGSWASGFLA ILILLFGFCA
     LIGNYYYGET NIGFLNKSKK LIFVYRIGVL AMIVFGCVAK VQLVWDLADL FMGLMVIVNL
     IAIFLLSKVV FTALKDYTRQ KKAGKDPVFY KDVLKNHNGI ECWPVSDTKT DTHNKQIS