GLNT_BACSU
ID GLNT_BACSU Reviewed; 478 AA.
AC O31464; Q7DL42;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Probable sodium/glutamine symporter GlnT;
GN Name=glnT; Synonyms=ybgH; OrderedLocusNames=BSU02420;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT Bacillus subtilis chromosome.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO N-TERMINUS.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP FUNCTION, AND GENE NAME.
RC STRAIN=168;
RX PubMed=15995196; DOI=10.1128/jb.187.14.4813-4821.2005;
RA Satomura T., Shimura D., Asai K., Sadaie Y., Hirooka K., Fujita Y.;
RT "Enhancement of glutamine utilization in Bacillus subtilis through the
RT GlnK-GlnL two-component regulatory system.";
RL J. Bacteriol. 187:4813-4821(2005).
CC -!- FUNCTION: Probably functions as a sodium/glutamine symporter for
CC glutamine uptake. {ECO:0000269|PubMed:15995196}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the alanine or glycine:cation symporter (AGCS)
CC (TC 2.A.25) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA33140.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB006424; BAA33140.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AL009126; CAB12036.2; -; Genomic_DNA.
DR PIR; C69751; C69751.
DR RefSeq; NP_388124.2; NC_000964.3.
DR RefSeq; WP_003246395.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; O31464; -.
DR SMR; O31464; -.
DR STRING; 224308.BSU02420; -.
DR PaxDb; O31464; -.
DR PRIDE; O31464; -.
DR EnsemblBacteria; CAB12036; CAB12036; BSU_02420.
DR GeneID; 938419; -.
DR KEGG; bsu:BSU02420; -.
DR PATRIC; fig|224308.179.peg.249; -.
DR eggNOG; COG1115; Bacteria.
DR InParanoid; O31464; -.
DR OMA; GMATKFV; -.
DR PhylomeDB; O31464; -.
DR BioCyc; BSUB:BSU02420-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015655; F:alanine:sodium symporter activity; IEA:InterPro.
DR InterPro; IPR001463; Na/Ala_symport.
DR PANTHER; PTHR30330; PTHR30330; 1.
DR Pfam; PF01235; Na_Ala_symp; 1.
DR PRINTS; PR00175; NAALASMPORT.
DR TIGRFAMs; TIGR00835; agcS; 1.
DR PROSITE; PS00873; NA_ALANINE_SYMP; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..478
FT /note="Probable sodium/glutamine symporter GlnT"
FT /id="PRO_0000161565"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 478 AA; 51584 MW; 334C8EDD16C244D6 CRC64;
MQQILEHIVG IANDLLWSKL LIVLLLSFGI YFTFRLKFLQ VRMLKEMVRV LREGAASRSK
NSISPFQAFC ISMAARVGTG NITGIAIAIA LGGPGAIFWM WIIAIIGSAS SFVESTLAQI
YKVKDVNGFR GGPAYYMEKG LNKRWMGALF AVLITLSFGI VFNSVQSNTV SLAFENAFGT
NRLTLGLILI AVFGTIIFGG VKRIAKLAES IVVVLAVLYI GVAFFVIFSN ITQLPGVLAL
IVKNAFGFDQ AAGGALGAAL MQGVRRGIFS NEAGMGSAPN AAATATTSHP VKQGLIQAFG
VLTDTLVICT STAFIILFSD AYHTPGLSGI ALTQASLSSH VGSWASGFLA ILILLFGFCA
LIGNYYYGET NIGFLNKSKK LIFVYRIGVL AMIVFGCVAK VQLVWDLADL FMGLMVIVNL
IAIFLLSKVV FTALKDYTRQ KKAGKDPVFY KDVLKNHNGI ECWPVSDTKT DTHNKQIS