ID   AMBI1_FISAU             Reviewed;         319 AA.
AC   V5TF65;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   19-FEB-2014, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=L-tryptophan isonitrile synthase AmbI1 {ECO:0000305};
DE            EC=4.1.99.25 {ECO:0000269|PubMed:24180436, ECO:0000269|PubMed:28212039};
GN   Name=ambI1 {ECO:0000303|PubMed:24180436};
GN   Synonyms=famH1 {ECO:0000303|PubMed:26629885};
OS   Fischerella ambigua (strain UTEX 1903).
OC   Bacteria; Cyanobacteria; Nostocales; Hapalosiphonaceae; Fischerella.
OX   NCBI_TaxID=230521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=UTEX 1903;
RX   PubMed=24180436; DOI=10.1021/cb400681n;
RA   Hillwig M.L., Zhu Q., Liu X.;
RT   "Biosynthesis of ambiguine indole alkaloids in cyanobacterium Fischerella
RT   ambigua.";
RL   ACS Chem. Biol. 9:372-377(2014).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UTEX 1903;
RX   PubMed=25198896; DOI=10.1186/s12866-014-0213-7;
RA   Micallef M.L., Sharma D., Bunn B.M., Gerwick L., Viswanathan R.,
RA   Moffitt M.C.;
RT   "Comparative analysis of hapalindole, ambiguine and welwitindolinone gene
RT   clusters and reconstitution of indole-isonitrile biosynthesis from
RT   cyanobacteria.";
RL   BMC Microbiol. 14:213-213(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UTEX 1903;
RX   PubMed=26629885; DOI=10.1021/jacs.5b10136;
RA   Li S., Lowell A.N., Yu F., Raveh A., Newmister S.A., Bair N., Schaub J.M.,
RA   Williams R.M., Sherman D.H.;
RT   "Hapalindole/ambiguine biogenesis is mediated by a cope rearrangement, C-C
RT   bond-forming cascade.";
RL   J. Am. Chem. Soc. 137:15366-15369(2015).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=28212039; DOI=10.1021/acs.orglett.7b00258;
RA   Chang W.C., Sanyal D., Huang J.L., Ittiamornkul K., Zhu Q., Liu X.;
RT   "In vitro stepwise reconstitution of amino acid derived vinyl isocyanide
RT   biosynthesis: detection of an elusive intermediate.";
RL   Org. Lett. 19:1208-1211(2017).
CC   -!- FUNCTION: Involved in the biosynthesis of ambiguines, a family of
CC       hapalindole-type alkaloids (PubMed:24180436). Responsible for the
CC       synthesis of the isonitrile group on tryptophan using ribulose 5-
CC       phosphate as the source of the carbon atom (PubMed:24180436,
CC       PubMed:28212039). {ECO:0000269|PubMed:24180436,
CC       ECO:0000269|PubMed:28212039}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-ribulose 5-phosphate + L-tryptophan = (2S)-3-(1H-indol-3-
CC         yl)-2-isocyanopropanoate + formaldehyde + H(+) + H2O + hydroxyacetone
CC         + phosphate; Xref=Rhea:RHEA:56696, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16842, ChEBI:CHEBI:27957,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57912, ChEBI:CHEBI:58121,
CC         ChEBI:CHEBI:140652; EC=4.1.99.25;
CC         Evidence={ECO:0000269|PubMed:24180436, ECO:0000269|PubMed:28212039};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:56697;
CC         Evidence={ECO:0000269|PubMed:24180436, ECO:0000269|PubMed:28212039};
CC   -!- SIMILARITY: Belongs to the isocyanide synthase family. {ECO:0000305}.
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DR   EMBL; KF664586; AHB62771.1; -; Genomic_DNA.
DR   EMBL; KJ742065; AIJ28556.1; -; Genomic_DNA.
DR   EMBL; KX451322; APB62257.1; -; Genomic_DNA.
DR   SMR; V5TF65; -.
DR   KEGG; ag:AHB62771; -.
DR   BioCyc; MetaCyc:MON-20401; -.
DR   BRENDA; 4.1.99.25; 15535.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR007817; Isocyanide_synthase_DIT1.
DR   InterPro; IPR017133; PvcA.
DR   PANTHER; PTHR37285; PTHR37285; 1.
DR   Pfam; PF05141; DIT1_PvcA; 1.
DR   PIRSF; PIRSF037196; Pyoverdine_chromoph_PvcA; 1.
PE   1: Evidence at protein level;
KW   Lyase.
FT   CHAIN           1..319
FT                   /note="L-tryptophan isonitrile synthase AmbI1"
FT                   /id="PRO_0000453964"
SQ   SEQUENCE   319 AA;  36821 MW;  C9EA3B347F4AEADB CRC64;
     MISEKILRHI FQYRRLLSDT EPCAKEPCSI CLAPHLPKIQ SFIENNEPIH FILPAFPAKS
     PNPQKVLGPM PDMGERVALQ FLQNLCNQIS EIYASGAKIT ICSDGRVFTD LVAITDENVS
     LYRQGIQRLL NEINADAIDT FCLENVFTGM SFDQMRKTLV KQYAQPIESI QERVNSEDKH
     RQFFKGIYHL LFDDYLVLYP DKSREQIEVE CNLRAYEVIQ RSNAWTTLVG QHFPQSLRLS
     IHPQDYHSNK IGIHMIKTSD QWGTPWHNAP MFNGKEFLLM KRKHIEDIGA SLVWHNDHPS
     HYILSEQVSQ ALVTLDNKS