AMBN_RAT
ID AMBN_RAT Reviewed; 422 AA.
AC Q62840; Q540J9; Q63043;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Ameloblastin;
DE Short=Amelin;
DE Flags: Precursor;
GN Name=Ambn;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Incisor;
RX PubMed=8626794; DOI=10.1074/jbc.271.8.4431;
RA Krebsbach P.H., Lee S.K., Matsuki Y., Kozak C.A., Yamada K.M., Yamada Y.;
RT "Full-length sequence, localization, and chromosomal mapping of
RT ameloblastin. A novel tooth-specific gene.";
RL J. Biol. Chem. 271:4431-4435(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=Sprague-Dawley; TISSUE=Tooth;
RX PubMed=8797107; DOI=10.1002/jbmr.5650110703;
RA Cerny R., Slaby I., Hammarstrom L., Wurtz T.;
RT "A novel gene expressed in rat ameloblasts codes for proteins with cell
RT binding domains.";
RL J. Bone Miner. Res. 11:883-891(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sprague-Dawley;
RA Lee S.K., Kim S.M., Lee Y.J., Yamada K.M., Yamada Y., Chi J.G.;
RT "Gene structure of rat ameloblastin and specific expression in
RT amelogenesis.";
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the mineralization and structural organization of
CC enamel.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q62840-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q62840-2; Sequence=VSP_000226;
CC -!- TISSUE SPECIFICITY: Ameloblast-specific.
CC -!- SIMILARITY: Belongs to the ameloblastin family. {ECO:0000305}.
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DR EMBL; U35097; AAC52428.1; -; mRNA.
DR EMBL; Z50083; CAA90414.1; -; mRNA.
DR EMBL; AY159302; AAN71737.1; -; Genomic_DNA.
DR EMBL; AY159295; AAN71737.1; JOINED; Genomic_DNA.
DR EMBL; AY159296; AAN71737.1; JOINED; Genomic_DNA.
DR EMBL; AY159297; AAN71737.1; JOINED; Genomic_DNA.
DR EMBL; AY159298; AAN71737.1; JOINED; Genomic_DNA.
DR EMBL; AY159299; AAN71737.1; JOINED; Genomic_DNA.
DR EMBL; AY159300; AAN71737.1; JOINED; Genomic_DNA.
DR RefSeq; NP_037032.1; NM_012900.1. [Q62840-1]
DR AlphaFoldDB; Q62840; -.
DR IntAct; Q62840; 8.
DR STRING; 10116.ENSRNOP00000005003; -.
DR GlyGen; Q62840; 1 site.
DR PaxDb; Q62840; -.
DR Ensembl; ENSRNOT00000105630; ENSRNOP00000087626; ENSRNOG00000003718. [Q62840-2]
DR GeneID; 25376; -.
DR KEGG; rno:25376; -.
DR UCSC; RGD:2101; rat. [Q62840-1]
DR CTD; 258; -.
DR RGD; 2101; Ambn.
DR VEuPathDB; HostDB:ENSRNOG00000003718; -.
DR eggNOG; ENOG502QWCP; Eukaryota.
DR GeneTree; ENSGT00390000018227; -.
DR HOGENOM; CLU_051782_0_0_1; -.
DR InParanoid; Q62840; -.
DR OMA; KPAMGGD; -.
DR OrthoDB; 1189806at2759; -.
DR PhylomeDB; Q62840; -.
DR TreeFam; TF337860; -.
DR Reactome; R-RNO-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-RNO-8957275; Post-translational protein phosphorylation.
DR PRO; PR:Q62840; -.
DR Proteomes; UP000002494; Chromosome 14.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0008083; F:growth factor activity; ISO:RGD.
DR GO; GO:0030345; F:structural constituent of tooth enamel; TAS:RGD.
DR GO; GO:0031214; P:biomineral tissue development; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; ISO:RGD.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:InterPro.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISO:RGD.
DR InterPro; IPR007798; Amelin.
DR PANTHER; PTHR14115; PTHR14115; 1.
DR Pfam; PF05111; Amelin; 1.
DR SMART; SM00817; Amelin; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Biomineralization; Extracellular matrix;
KW Glycoprotein; Hydroxylation; Phosphoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..422
FT /note="Ameloblastin"
FT /id="PRO_0000001195"
FT REGION 271..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 42
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250"
FT MOD_RES 48
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q28989"
FT CARBOHYD 117
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 104..118
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8797107"
FT /id="VSP_000226"
FT CONFLICT 324
FT /note="M -> I (in Ref. 2; CAA90414)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 422 AA; 45206 MW; 0F983772E9082A89 CRC64;
MSASKIPLFK MKGLLLFLSL VKMSLAVPAF PQQPGAQGMA PPGMASLSLE TMRQLGSLQG
LNALSQYSRL GFGKALNSLW LHGLLPPHNS FPWIGPREHE TQQYEYSLPV HPPPLPSQPS
LQPHQPGLKP FLQPTAATGV QVTPQKPGPH PPMHPGQLPL QEGELIAPDE PQVAPSENPP
TPEVPIMDFA DPQFPTVFQI AHSLSRGPMA HNKVPTFYPG MFYMSYGANQ LNAPARIGFM
SSEEMPGERG SPMAYGTLFP GYGGFRQTLR GLNQNSPKGG DFTVEVDSPV SVTKGPEKGE
GPEGSPLQEA SPDKGENPAL LSQMAPGAHA GLLAFPNDHI PNMARGPAGQ RLLGVTPAAA
DPLITPELAE VYETYGADVT TPLGDGEATM DITMSPDTQQ PPMPGNKVHQ PQVHNAWRFQ
EP
