ID   AMC2_PIG                Reviewed;         117 AA.
AC   P22952;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Alveolar macrophage chemotactic factor 2;
DE   AltName: Full=Alveolar macrophage chemotactic factor II;
DE            Short=AMCF-II;
DE   Flags: Precursor;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 37-66.
RC   TISSUE=Lung;
RX   PubMed=1420165; DOI=10.1021/bi00158a011;
RA   Goodman R.B., Foster D.C., Mathewes S.L., Osborn S.G., Kuijper J.L.,
RA   Forstrom J.W., Martin T.R.;
RT   "Molecular cloning of porcine alveolar macrophage-derived neutrophil
RT   chemotactic factors I and II; identification of porcine IL-8 and another
RT   intercrine-alpha protein.";
RL   Biochemistry 31:10483-10490(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 37-66.
RC   STRAIN=Yorkshire;
RX   PubMed=1850745; DOI=10.1016/s0021-9258(18)92996-0;
RA   Goodman R.B., Forstrom J.W., Osborn S.G., Chi E.Y., Martin T.R.;
RT   "Identification of two neutrophil chemotactic peptides produced by porcine
RT   alveolar macrophages.";
RL   J. Biol. Chem. 266:8455-8463(1991).
CC   -!- FUNCTION: Has chemotactic activity for porcine, and in a lesser extent,
CC       for human neutrophils.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Alveolar macrophages.
CC   -!- INDUCTION: By lipopolysaccharide (LPS).
CC   -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC       {ECO:0000305}.
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DR   EMBL; M99368; AAA30991.1; -; mRNA.
DR   PIR; B39819; B39819.
DR   PIR; B44253; B44253.
DR   RefSeq; NP_999041.1; NM_213876.1.
DR   AlphaFoldDB; P22952; -.
DR   SMR; P22952; -.
DR   STRING; 9823.ENSSSCP00000009558; -.
DR   PaxDb; P22952; -.
DR   PRIDE; P22952; -.
DR   Ensembl; ENSSSCT00000009811; ENSSSCP00000009558; ENSSSCG00000008957.
DR   Ensembl; ENSSSCT00005065321; ENSSSCP00005040365; ENSSSCG00005040852.
DR   Ensembl; ENSSSCT00015038230; ENSSSCP00015015167; ENSSSCG00015028811.
DR   Ensembl; ENSSSCT00030051003; ENSSSCP00030023206; ENSSSCG00030036680.
DR   Ensembl; ENSSSCT00045017028; ENSSSCP00045011741; ENSSSCG00045010048.
DR   Ensembl; ENSSSCT00050010342; ENSSSCP00050004426; ENSSSCG00050007593.
DR   Ensembl; ENSSSCT00055061077; ENSSSCP00055048966; ENSSSCG00055030642.
DR   Ensembl; ENSSSCT00060054400; ENSSSCP00060023195; ENSSSCG00060040182.
DR   Ensembl; ENSSSCT00065019309; ENSSSCP00065007906; ENSSSCG00065014514.
DR   Ensembl; ENSSSCT00070012150; ENSSSCP00070009998; ENSSSCG00070006348.
DR   GeneID; 396900; -.
DR   KEGG; ssc:396900; -.
DR   CTD; 396900; -.
DR   eggNOG; ENOG502S7MM; Eukaryota.
DR   GeneTree; ENSGT00940000162749; -.
DR   HOGENOM; CLU_143902_1_0_1; -.
DR   InParanoid; P22952; -.
DR   OMA; RELRCNC; -.
DR   OrthoDB; 1556509at2759; -.
DR   TreeFam; TF333433; -.
DR   Reactome; R-SSC-380108; Chemokine receptors bind chemokines.
DR   Reactome; R-SSC-418594; G alpha (i) signalling events.
DR   Proteomes; UP000008227; Chromosome 8.
DR   Proteomes; UP000314985; Chromosome 8.
DR   Bgee; ENSSSCG00000008957; Expressed in ileum and 12 other tissues.
DR   Genevisible; P22952; SS.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR   GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0042119; P:neutrophil activation; IEA:Ensembl.
DR   GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR   CDD; cd00273; Chemokine_CXC; 1.
DR   InterPro; IPR001089; Chemokine_CXC.
DR   InterPro; IPR018048; Chemokine_CXC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR033899; CXC_Chemokine_domain.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR10179; PTHR10179; 1.
DR   Pfam; PF00048; IL8; 1.
DR   PRINTS; PR00437; SMALLCYTKCXC.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; SSF54117; 1.
DR   PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE   1: Evidence at protein level;
KW   Chemotaxis; Cytokine; Direct protein sequencing; Disulfide bond;
KW   Inflammatory response; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000269|PubMed:1420165,
FT                   ECO:0000269|PubMed:1850745"
FT   CHAIN           37..117
FT                   /note="Alveolar macrophage chemotactic factor 2"
FT                   /id="PRO_0000005139"
FT   DISULFID        52..78
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..94
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   117 AA;  12343 MW;  CD3D27410934F910 CRC64;
     MRLLTSRATR VPSPSGLLCA VLAMLLLTPS GPLASASPIE AAEAAVVREL RCMCLTTTPG
     IHPKMISDLQ VIPAGPQCSK AEVIATLKNG KEVCLDPKAP LIKKIVQKML DSGKKKN