ID   AMCI_APIME              Reviewed;          56 AA.
AC   P56682;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Chymotrypsin inhibitor;
DE   AltName: Full=AMCI;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1]
RP   PROTEIN SEQUENCE, AND STRUCTURE BY NMR.
RC   TISSUE=Hemolymph;
RX   PubMed=10411628; DOI=10.1046/j.1432-1327.1999.00406.x;
RA   Bania J., Stachowiak D., Polanowski A.;
RT   "Primary structure and properties of the cathepsin G/chymotrypsin inhibitor
RT   from the larval hemolymph of Apis mellifera.";
RL   Eur. J. Biochem. 262:680-687(1999).
CC   -!- FUNCTION: Chymotrypsin and cathepsin G inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the serine protease inhibitor-like (TIL domain-
CC       containing) family. {ECO:0000305}.
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DR   PDB; 1CCV; NMR; -; A=1-56.
DR   PDBsum; 1CCV; -.
DR   AlphaFoldDB; P56682; -.
DR   BMRB; P56682; -.
DR   SMR; P56682; -.
DR   STRING; 7460.GB50121-PA; -.
DR   MEROPS; I08.008; -.
DR   PaxDb; P56682; -.
DR   eggNOG; ENOG502SWNJ; Eukaryota.
DR   EvolutionaryTrace; P56682; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036084; Ser_inhib-like_sf.
DR   InterPro; IPR002919; TIL_dom.
DR   Pfam; PF01826; TIL; 1.
DR   SUPFAM; SSF57567; SSF57567; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..56
FT                   /note="Chymotrypsin inhibitor"
FT                   /id="PRO_0000174334"
FT   DOMAIN          3..56
FT                   /note="TIL"
FT   DISULFID        3..36
FT   DISULFID        12..32
FT   DISULFID        16..28
FT   DISULFID        20..56
FT   DISULFID        38..50
FT   STRAND          7..13
FT                   /evidence="ECO:0007829|PDB:1CCV"
FT   STRAND          28..31
FT                   /evidence="ECO:0007829|PDB:1CCV"
FT   STRAND          33..38
FT                   /evidence="ECO:0007829|PDB:1CCV"
FT   STRAND          43..45
FT                   /evidence="ECO:0007829|PDB:1CCV"
FT   TURN            46..48
FT                   /evidence="ECO:0007829|PDB:1CCV"
FT   STRAND          49..51
FT                   /evidence="ECO:0007829|PDB:1CCV"
FT   HELIX           53..55
FT                   /evidence="ECO:0007829|PDB:1CCV"
SQ   SEQUENCE   56 AA;  5973 MW;  092B2815AE6B2B7F CRC64;
     EECGPNEVFN TCGSACAPTC AQPKTRICTM QCRIGCQCQE GFLRNGEGAC VLPENC