ID   AMDA_MYCSM              Reviewed;         406 AA.
AC   Q07838;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Acetamidase;
DE            EC=3.5.1.4;
GN   Name=amdA;
OS   Mycolicibacterium smegmatis (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1772;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-24; 82-101 AND
RP   181-195.
RC   STRAIN=ATCC 19420 / DSM 43756 / JCM 5866 / KCTC 9108 / NCTC 8159 / NRRL
RC   B-14616 / Cornell 3;
RX   PubMed=8473863; DOI=10.1099/00221287-139-3-575;
RA   Mahenthiralingam E., Draper P., Davis E.O., Colston M.J.;
RT   "Cloning and sequencing of the gene which encodes the highly inducible
RT   acetamidase of Mycobacterium smegmatis.";
RL   J. Gen. Microbiol. 139:575-583(1993).
CC   -!- FUNCTION: Allows acetamide to be used as a sole carbon or nitrogen
CC       source.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetamide + H2O = acetate + NH4(+); Xref=Rhea:RHEA:45048,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:27856, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30089; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the acetamidase/formamidase family.
CC       {ECO:0000305}.
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DR   EMBL; X57175; CAA40462.1; -; Genomic_DNA.
DR   PIR; A47696; A47696.
DR   AlphaFoldDB; Q07838; -.
DR   SMR; Q07838; -.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR004304; FmdA_AmdA.
DR   PANTHER; PTHR31891; PTHR31891; 1.
DR   Pfam; PF03069; FmdA_AmdA; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..406
FT                   /note="Acetamidase"
FT                   /id="PRO_0000064577"
FT   REGION          387..406
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   406 AA;  43964 MW;  235508C2DE36615D CRC64;
     MPEVVFSVDH SKSMRDQAVP GHNRWHPDIP AAATVKPGSE FRIECKEWTD GQIGNNDSAN
     DVRDVDLAPC HMLSGPIKVE GAEPGDLLIV DILDIGPVPQ TNGPNCGEGW GYSGIFAKVN
     GGGFLTDYYP DAYKAIWDFH GQQCTSRHVP GVRYTGITHP GLFGTAPSPD LLAKWNERER
     ALIATDPDRV PPLALPPLVD GTLGGTASGD LLQAIANDGA RTVPPRENGG NHDIKNFTRG
     SRIFYPVFVE GAMLSGGDLH FSQGDGEINF CGAIEMGGFI DMHVDLIKGG METYGVTTNP
     IFMPGRVEPL YSEWLTFIGI SVDHAENRNA YMDATMAYRN ACLNAIEYLK KWGYTGEQAY
     LILGTSPIEG ASAASWTSRT HVVRCSCRPR SSTSTSPRRQ GPAEGR